Replication factor C subunit 4 is a
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...
that in humans is encoded by the ''RFC4''
gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...
.
Function
The elongation of
primed DNA templates by
DNA polymerase delta
DNA polymerase delta (DNA Pol δ) is an enzyme complex found in eukaryotes that is involved in DNA replication and repair. The DNA polymerase delta complex consists of 4 subunits: POLD1, POLD2, POLD3, and POLD4. DNA Pol δ is an enzyme used for ...
and
DNA polymerase epsilon
DNA polymerase epsilon is a member of the DNA polymerase family of enzymes found in eukaryotes. It is composed of the following four subunits: POLE (central catalytic unit), POLE2 (subunit 2), POLE3 (subunit 3), and POLE4 (subunit 4). Recent evi ...
requires the accessory proteins
proliferating cell nuclear antigen
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, whe ...
(PCNA) and
replication factor C
The replication factor C, or RFC, is a five-subunit protein complex that is required for DNA replication.
The subunits of this heteropentamer are named Rfc1, Rfc2, Rfc3, Rfc4, and Rfc5 in ''Saccharomyces cerevisiae''. RFC is used in eukaryotic re ...
(RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses
DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
Alternatively spliced
Alternative splicing, or alternative RNA splicing, or differential splicing, is an alternative splicing process during gene expression that allows a single gene to code for multiple proteins. In this process, particular exons of a gene may be in ...
transcript variants encoding the same protein have been reported.
Interactions
RFC4 has been shown to
interact
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with:
*
BRD4
Bromodomain-containing protein 4 is a protein that in humans is encoded by the ''BRD4'' gene.
BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET famil ...
,
*
CHTF18
Chromosome transmission fidelity protein 18 homolog is a protein that in humans is encoded by the ''CHTF18'' gene.
Interactions
CHTF18 has been shown to interact with:
* DCC1,
* PCNA,
* RFC2,
* RFC3,
* RFC4, and
* RFC5
Replication factor ...
,
*
PCNA
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, whe ...
,
* RFC2
Replication factor C subunit 2 is a protein that in humans is encoded by the ''RFC2'' gene.
Function
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell n ...
,
* RFC3
Replication factor C subunit 3 is a protein that in humans is encoded by the ''RFC3'' gene.
Function
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell n ...
, and
* RFC5
Replication factor C subunit 5 is a protein that in humans is encoded by the ''RFC5'' gene.
Function
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell ...
.
References
Further reading
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