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Bacterial initiation factor-2 is a bacterial initiation factor. IF2 binds to an initiator tRNA and controls the entry of tRNA onto the ribosome. IF2, bound to GTP, binds to the
30S The prokaryotic small ribosomal subunit, or 30Svedberg, S subunit, is the smaller subunit of the 70S ribosome found in prokaryotes. It is a complex of the 16S ribosomal RNA (rRNA) and 19 proteins. This complex is implicated in the binding of tr ...
P site. After associating with the 30S subunit, fMet-tRNAf binds to the IF2 then IF2 transfers the tRNA into the partial P site. When the 50S subunit joins, it hydrolyzes GTP to GDP and Pi, causing a conformational change in the IF2 that causes IF2 to release and allow the 70S ribosome to form. Human mitochondria use a nuclear-encoded homolog, MTIF2, for translation initiation.


References

{{GTPases Protein biosynthesis Gene expression