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Ponceau 2R, Xylidine ponceau, Ponceau G, Red R, Acid Red 26, Food Red 5, or C.I. 16150 is a red azo dye used in
histology Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures vi ...
for staining. It is easily soluble in water and slightly in
ethanol Ethanol (abbr. EtOH; also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound. It is an alcohol with the chemical formula . Its formula can be also written as or (an ethyl group linked to a ...
. It usually comes as a disodium salt. Xylidine ponceau is mostly used only in the
Masson's trichrome Masson's trichrome is a three-colour staining procedure used in histology. The recipes evolved from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells ...
stain as a red
counterstain A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope. Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique ...
, where it imparts orange hue to the red-stained
cytoplasm In cell biology, the cytoplasm is all of the material within a eukaryotic cell, enclosed by the cell membrane, except for the cell nucleus. The material inside the nucleus and contained within the nuclear membrane is termed the nucleoplasm. ...
ic structures. Its other names are: Acetacid red J, Acidal ponceau G, Acid leather red KPR, Acid leather red P2R, Acid leather scarlet IRW, Acid ponceau R, Acid ponceau 2RL, Acid ponceau special, Acid scarlet, Acid scarlet 2RL, Acid scarlet 2RN, Acilan ponceau RRL, Ahcocid fast scarlet R, Aizen ponceau RH, Amacid lake scarlet 2R, Amacid scarlet 2R, Brilliant ponceau G, Calcocid 2RIL, Calcocid scarlet 2R, Calcocid scarlet 2RIL, Calcolake scarlet 2R, Certicol ponceau MXS, C.I. Acid red 26, C.I. Food red 5, Colacid ponceau special, Comacid scarlet 2R D and C red No. 5, Edicol supra ponceau R, Fenazo scarlet 2R, Hexacol ponceau MX, Hexacol ponceau 2R, Hidacid scarlet 2R, Hispacid ponceau R, Java ponceau 2R, Kiton ponceau R, Kiton scarlet 2RC, Lake ponceau, Lake scarlet R, Lake scarlet 2RBN, Naphthalene lake scarlet R, Naphthalene scarlet R, Naphthazine scarlet 2R, Neklacid red RR, New ponceau 4R, Paper red HRR, Pigment ponceau R, Ponceau BNA, Ponceau de xylidine, Ponceau R, Ponceau red, Ponceau RG, Ponceau xylidine, 1695 Red, Red for lakes J, Red R, Tertracid ponceau 2R, Xylidine ponceau 3RS, Xylidine red.


References

Azo dyes Staining dyes Naphthalenesulfonates 2-Naphthols {{cell-biology-stub