Lifecycle
The lifecycle of ''P. tenuis'' is complex and multistaged. Adults lay eggs on the dura mater (the outer layer of the meninges) of the brain or directly into the bloodstream of an infected host. The eggs hatch into first-stage larvae, which travel in the bloodstream to the lungs, where they travel up the respiratory tract, are swallowed, and then pass out of the body in the mucus coating of fecal pellets. Gastropods such as snails and slugs feed upon the mucus coating of the fecal pellets and ingest the larvae. While in the gastropod, the larvae develop into second- and third-stage larvae, which are capable of infection. Gastropods carrying second- and third-stage larvae may be accidentally ingested with plants, which results in the larvae being transmitted to a new host. The larvae then move into the new host's stomach wall and make their way to the CNS, as in white-tailed deer, or the brain as in other ungulates. Once in these tissues, they develop into their adult third stage of life and lay eggs to begin the cycle again.Transmission
Adults of ''P. tenuis'' can persist for many years in a single host, which allows for many first-stage larvae to be shed in feces. It is quite common in many populations of white-tailed deer, which have built up a strong resistance. After gastropod ingestion, moose or other deer may be hosts of the second- and third-stage worms. Moose resistance to ''P. tenuis'' is much lower than white-tailed deer, which results in a higher mortality rate. Infected deer density, temperature, climate conditions, and length of transmission periods all affect transmission levels. * Areas with higher deer populations experience dramatic increases in incidental brainworm larvae consumption. *The temperature and climate conditions of summers can regulate gastropod abundance, with hot, dry summers reducing populations and cool, dry summers increasing populations. *High or low gastropod populations directly affect the transmission rate of larval worms to new hosts. Hot, dry summers also reduce the survival numbers of first-stage larvae on fecal pellets. *Variation of transmission periods is based on the time when snow is absent from the ground. Gastropods are dormant during snow periods, thus first-stage worms cannot be transmitted to additional hosts. So, shorter seasons of snow are met with longer transmission periods of increased potential for transmission through the intermediate vector gastropods.Symptoms/clinical signs
Brainworm affects neurological and behavioral responses. Deer rarely show any external symptoms of'' P. tenius'' infection due to their high acquired resistance. Moose, however, have low resistance, and may show a number of symptoms. Though infrequent, cases of moose recovering from brainworm infection have been reported. In both deer and moose, symptom severity does not necessarily vary with severity of infection. *Infected individuals may not have any external symptoms. *Mild symptoms may include slower movements and response time, frequent stumbling, unusually tilted head, and emaciation. *Severe symptoms include extreme weakness, lameness, walking in circles, partial or whole blindness, loss of fear for humans, ataxia, and mortality. Several other ungulates are susceptible to brainworm infection, including elk, caribou, mule deer, sheep, goats, alpacas, rarely cattle, and rarely horses. Severe neurological damage similar to that of infected moose is shown to occur in these species.Diagnosis
Presently, a commercial antibody test that can detect ''P. tenuis'' antemortem does not exist. Diagnosis in deer can be conducted by analyzing fecal pellets for larval ''P. tenuis'', or ''post mortem'' necropsy to detect presence of adult ''P. tenuis'' in the brain cavity or second- and third-stage worms along the spinal cord. However, brainworm larvae are difficult to distinguish from other parasitic worm species that can also be found in fecal pellets, so detection of adult worms through necropsy is recommended. Diagnosis in moose is conducted with necropsy to detect worms in the brain or spinal cord. Diagnosis in horses can be conducted with postmortem samples used for polymerase chain reaction (PCR) testing. Positive PCR results show evidence of ''P.'' ''tenuis'' infecting equines. In veterinary patients, such as goats, an eosinophilic pleocytosis can be suggestive of ''P. tenuis'' infection.History
In 1912, an unknown neurological disease affecting moose was first reported in Minnesota. Major declines in the moose population were reported, 1925–27 and 1933–34, following the discovery of this unknown disease. In 1963, a meningeal brainworm, ''Pneumostrongylus tenuis'', was determined as the etiological agent causing neurological disease in moose. Around 1971, taxonomists reclassified it as ''Parelaphostrongylus tenuis''. White-tailed deer populations in the eastern United States are now known to be commonly infected with meningeal worm. Infection in the southeastern and western United States is less common. This disease has also been found in the Canadian provinces of Ontario, Saskatchewan, Manitoba, and Nova Scotia. The grassland biomes of the United States and Canada apparently act as a barrier to the movement of the disease. Evidence from Canada seems to support this, since the western distribution of meningeal worm has changed little since the 1960s.Epidemiology
The geographic ranges of moose and white-tailed deer were historically separate prior to the 20th century. Moose are well adapted to winter survival, whereas deer are not. They could not withstand the harsh winters in these regions of the northeastern United States and the southeastern provinces of Canada. Deer populations began to move into the southern portions of moose range in the early 1900s following changes in climate, logging, mining, forest fires, and increasing human development. High-quality deer habitat associated with these changes has led to dramatic increases in deer abundance in these regions. White-tailed deer are the normal host of the ''P. tenuis'' parasite and are immunologically adapted to its presence. Deer and ''P. tenuis'' have coadapted in an evolutionary arms race over time. Deer remain largely unaffected by the presence of ''P. tenuis'' because of the immunity they have built as a result of coadaptation. The prevalence and infection rate of ''P. tenuis'' in deer is density dependent; increased rates of infection by the parasite are the result of higher deer densities. Moose populations on the southern fringes of their range have recently experienced dramatic declines. As deer encroached upon the southern fringes of moose range, they introduced the parasite to aTreatment
Currently, there is no definitive treatment for ''P. tenuis'' in mammals, though research is still being conducted''.'' The use of anthelmintics (ivermectin and fenbendazole) have been attempted in white-tailed deer. The results indicate, however, that ivermectin was ineffective against larvae that had already reached the spinal cord. Fenbendazole and ivermectin, combined with anti-inflammatory therapy have been used to manage infections in goats.Current and past research
The majority of moose brainworm research in North America has been conducted in northern Minnesota, where a historical moose population of 4,000 to 5,000 moose had declined to an estimated 1,200 individuals by 1997. In this region, wildlife managers are challenged to predict and attempt to mitigate moose declines resulting from deer-related pathogens. Because no effective methods to prevent the transmission or infection of brainworm in moose have been found, managers have focused their efforts on decreasing deer densities in these regions. The Minnesota DNR identified that population densities greater than 12 deer/square mile result in increased moose mortality as a result of brainworm. Managers in these areas are responsible for evaluating suitable habitat for moose and deer, as well as setting management priorities and population goals to decrease the transmission of ''Parelaphostrongylus tenuis'' in moose. Though the main focus of research has been on white-tailed deer and moose, research has found that the guinea pig can be used as an experimental model of ''P. tenuis'' infection.References
{{Taxonbar, from=Q7136712 Ruminant diseases Parasitic nematodes of mammals Nematodes described in 1945 Strongylida