PBLU Plasmid Map
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pBLU is a commercially produced bacterial
plasmid A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; how ...
that contains genes for ampicillin resistance ( beta lactamase and beta galactosidase). It is often used in conjunction with an ampicillin-susceptible ''
E. coli ''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus ''Escher ...
'' strain to teach students about transformation of eubacteria. It is 5,437 base pairs long. There is a multiple cloning site in the ''lacZ'' gene.


References

Molecular biology techniques Plasmids {{biotech-stub