The PRD1 Phage P35 Holin (P35 Holin) Family
TC# 1.E.5 is a member of
Holin Superfamily III.
The prototype for this family is the lipid-containing PRD1 enterobacterial phage holin protein P35 (12.8
kDaTC# 1.E.5.1.1 encoded by gene ''XXXV'' (''orfT'').
It is a component of a typical holin-endolysin system which functions to
lyse Lyse may refer to:
* Lyse Abbey, a former Cistercian abbey in Norway
* Lyse, an alternative name of Lysebotn, Norway
* Lyse Energi, a Norwegian power company
* Łyse, Masovian Voivodeship, a village in east-central Poland
* Łyse, Podlaskie Voivode ...
the host bacterial cell.
Structure
P35 holin
TC# 1.E.5.1.1 has 3 transmembrane segments (TMSs) with 5 positively charged residues between TMSs 1 and 2. It has 4 positively charged residues at the
C-terminus
The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH). When the protein is ...
.
It is therefore thought that the
N-terminus
The N-terminus (also known as the amino-terminus, NH2-terminus, N-terminal end or amine-terminus) is the start of a protein or polypeptide, referring to the free amine group (-NH2) located at the end of a polypeptide. Within a peptide, the ami ...
is in the periplasm and the C-terminus is in the cytoplasm. Homologues of 109 amino acyl residues (aas), which also have 3 putative TMSs, are encoded in the genomes of ''
Xylella fastidiosa'' strains.
Function
The reaction catalyzed by P35 holin is:
: autolysin (in) → autolysin (out)
See also
*
Bacteriophage
A bacteriophage (), also known informally as a ''phage'' (), is a duplodnaviria virus that infects and replicates within bacteria and archaea. The term was derived from "bacteria" and the Greek φαγεῖν ('), meaning "to devour". Bacteri ...
*
Phage typing
Phage typing is a Phenotype, phenotypic method that uses Bacteriophage, bacteriophages ("phages" for short) for detecting and identifying single Strain (biology), strains of bacteria. Phages are viruses that infect bacteria and may lead to bacteria ...
*
Holin
*
Lysin
*
Transporter Classification Database
References
Holins
Protein families
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