''Oropouche orthobunyavirus'' (OROV) is one of the most common

orthobunyavirus ''Orthobunyavirus'' is a genus of the '' Peribunyaviridae'' family in the order ''Bunyavirales''. There are currently ~170 viruses recognised in this genus. These have been assembled into 103 species and 20 serogroups. The name ''Orthobunyavirus ...

es. When OROV infects humans, it causes a rapid fever illness called

Oropouche fever Oropouche fever is a tropical viral infection transmitted by biting midges and mosquitoes from the blood of sloths to humans. This disease is named after the region where it was first discovered and isolated at the Trinidad Regional Virus Laborat ...

. OROV was originally reported in Trinidad and Tobago in 1955 from the blood sample of a fever patient and from a pool of '' Coquillettidia venezuelensis'' mosquitoes. In 1960, OROV was isolated from a sloth (''

Bradypus tridactylus The pale-throated sloth (''Bradypus tridactylus''), occasionally known as the ai (), is a species of three-toed sloth that inhabits tropical rainforests in northern South America. It is similar in appearance to, and often confused with, the brow ...

'') and a pool of '' Ochlerotatus serratus'' mosquitoes in Brazil. The virus is considered a public health threat in tropical and subtropical areas of Central and South America, with over half a million infected people as of 2005. OROV is considered to be an arbovirus due to the method of transmission by the mosquitoes '' Aedes serratus'' and ''

Culex quinquefasciatus ''Culex'' is a genus of mosquitoes, several species of which serve as vectors of one or more important diseases of birds, humans, and other animals. The diseases they vector include arbovirus infections such as West Nile virus, Japanese encep ...

'' among sloths, marsupials, primates, and birds.

Epidemic sites

Between 1961 and 1980, OROV was reported in the northern state of Pará, Brazil, and from 1980 to 2004, OROV had spread to the Amazonas, Amapá, Acre, Rondônia, Tocantis, and Maranhão. The virus causes Oropouche fever, an urban arboviral disease that has since resulted in >30 epidemics during 1960–2009.

Virology

Currently, based on the small segment (SRNA) genetic information, there are 4 major genotypes (I–IV) of OROV. Genotype I was isolated from strains in Acre, Amazonas, Maranhão, Tocantis, Pará, Trinidad, and Tobago. Genotype II was obtained during the spread in Amapá, Pará, Rondônia, and Peru. Genotype III was isolated from samples in Acre, Minas Gerais, Panama, and Rondônia. The final genotype IV was isolated from Amazonas.

Dispersion

A possible dispersal could be predicted for the four genotypes based on time-scaled analysis and epidemiologic data association. Genotype I possibly dispersed towards western Pará, Trinidad, and Tobago. After, genotype I progressed towards Amazonas, Acre, Maranhao, and Tocantins. Genotype II possibly emerged in Amapá, Pará, Rondônia, and Peru at the same time. Genotype III emerged in Rondônia, moved towards Panama, Acre, and Maranhão. From Maranhão, the genotype progressed towards Minas Gerais. Finally, genotype IV emerged from the city of Manaus and Amazonas.

Experimentation and research

OROV has been used extensively in testing with

HeLa cells HeLa (; also Hela or hela) is an immortalized cell line used in scientific research. It is the oldest and most commonly used human cell line. The line is derived from cervical cancer cells taken on February 8, 1951, named after Henrietta L ...

to study the mechanisms of apoptosis induced by the virus. It was found that OROV causes apoptosis by DNA fragmentation. In UV-inactivated OROV, virus-receptor binding was not enough and that viral uncoating and replication were needed to induce apoptosis.

References

{{Taxonbar, from=Q3560891 Orthobunyaviruses