Nucleobindin-1 (NUCB1), also known as calnuc, is a
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...
that in humans is encoded by the ''NUCB1''
gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...
.
Structure
The human calnuc protein contains 461 amino acids and has a pI of 4.9.
The protein contains the following regions and
domain
Domain may refer to:
Mathematics
*Domain of a function, the set of input values for which the (total) function is defined
**Domain of definition of a partial function
**Natural domain of a partial function
**Domain of holomorphy of a function
* Do ...
s:
*
Signal peptide
A signal peptide (sometimes referred to as signal sequence, targeting signal, localization signal, localization sequence, transit peptide, leader sequence or leader peptide) is a short peptide (usually 16-30 amino acids long) present at the N-ter ...
*
Leucine-zipper The leucine-zipper of calnuc may be responsible for its dimerization.
* aa 347-389 –
Nuclear localization signal A nuclear localization signal ''or'' sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport. Typically, this signal consists of one or more short sequences of positively charged lysines o ...
* 2 zinc binding sites
with high affinity (K
d = 20 nM)
*
carboxypeptidase
A carboxypeptidase ( EC number 3.4.16 - 3.4.18) is a protease enzyme that hydrolyzes (cleaves) a peptide bond at the carboxy-terminal (C-terminal) end of a protein or peptide. This is in contrast to an aminopeptidases, which cleave peptide bonds ...
-like motifs : HFREXnH (aa 66) and HFTEXnH (aa 146)
* 2
EF-hands
* Potential anchor region
– 15-aa hydrophobic region at its COOH terminus that may be sufficient for membrane anchoring
References
Further reading
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EF-hand-containing proteins
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