The NhaE family
TC# 2.A.111
belongs to the Ion Transporter (IT) Superfamily, which has an end. A representative list of proteins belonging to the NhaE family can be found in th
Transporter Classification Database
The NhaH family consists of proteins from Gram-negative bacteria (e.g., ''

Leptospira ''Leptospira'' ( grc, leptos, italics=yes, 'fine, thin' and la, spira, links=no, 'coil') is a genus of spirochaete bacteria, including a small number of pathogenic and saprophytic species. ''Leptospira'' was first observed in 1907 in kidney t ...

'', '' Azotobacter'', '' Neisseria'', '' Ralstonia'', ''

Chlorobium ''Chlorobium'' is a genus of green sulfur bacteria. They are photolithotrophic oxidizers of sulfur and most notably utilise a noncyclic electron transport chain to reduce NAD+. Photosynthesis is achieved using a Type 1 Reaction Centre using ba ...

'' and ''

Rhizobia Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). To express genes for nitrogen fixation, rhizobia require a plant host; they cannot independently fix nitrogen. In gene ...

l'' species). The proteins are of about 480 aas with 12-14 putative TMSs. An

open reading frame In molecular biology, open reading frames (ORFs) are defined as spans of DNA sequence between the start and stop codons. Usually, this is considered within a studied region of a prokaryotic DNA sequence, where only one of the six possible readin ...

(ORF) from the genome of '' Neisseria meningitidis'' displaying similarity with the NhaE type of Na⁺/H⁺ antiporters was expressed in ''E. coli'' and characterized for sodium transport ability. The ''N. meningitidis'' antiporter (NmNhaE) was able to complement an ''E. coli'' strain devoid of Na⁺/H⁺ antiporters (KNabc) with respect to the ability to grow in the presence of high concentrations of NaCl or LiCl. Ion transport assays in everted vesicles prepared from the KNabc strain expressing NmNhaE from a plasmid confirmed its ability to translocate Na⁺ and Li⁺. The generalized reaction catalyzed by NhaE is,

Na⁺ or Li⁺ (in) + H⁺ (out) ⇌ Na⁺ or Li⁺ (out) + H⁺ (in)

References

Integral membrane proteins Membrane proteins Protein families Transmembrane proteins Transmembrane transporters Transport proteins {{membrane-protein-stub

See also

References