Nephroselmis

''Nephroselmis'' is a genus of green algae. It has been placed in the family Nephroselmidaceae, although a 2009 study suggests that it should be separated into its own class, Nephroselmidophyceae. One species can be an endosymbiont of ''Hatena arenicola''.

Morphology

The cell body is right-left flattened. On the ventral side are two heterodynamic unequal flagella, the shorter beats towards the anterior direction while the long trails behind. ''Nephroselmis'' have a single cup-shaped chloroplast that contains an eyespot in its anterior-ventral edge below the short flagellum and a pyrenoid with starch plates. Vacuole is located in the left-anterior side, nucleus is located in the right-posterior side. The flagellar root system consists of a rhizoplast and three microtubular roots, one of which is multilayered.

All the cells surface area is covered with layers of unminerallized scales, while the surface of the flagella has hairs as well. The cell body surface is covered by two to four layers of body scales. Flagella are covered with at least two scale layers, the second is characteristically rod-shaped. On top of that ''Nephroselmis'' has either a third layer of flagellar scales or flagellar pit scales.

The scales morphology can be an important taxonomic characteristic for differentiating between the species. In the case of ''Nephroselmis spinosa,'' that exhibits some distinctive morphological characteristics, one of them is a unique outer scale type that has a spine that is extended for about 1μm, is slightly curved and has a hook shape at the end.

Symbiosis with ''

Hatena arenicola''

A unique symbiosis occurs between the katablepharid ''Hatena arenicola'' and ''Nephroselmis rotunda.'' One of the interpretations to the observations of their interaction is that this symbiosis stage is leading to a secondary endosymbiosis, which will produce a completely new life form.

This symbiosis is distinct from other reported cases of ongoing symbiosis in that the symbiont plastid is selectively enlarged up to ten times than the normal size.

There is a possibility that retention of Nephroselmis-derived compartments is a precondition of the cell division process of ''H. arenicola'', due to the fact that cell division was only observed in ''H. arenicola'' individuals after they have incorporated the ''N. rotunda'' parts into their cell body.

Ecological Aspects

The genus ''Nephroselmis'' according to reports and documentations done until 2009 appears to have global worldwide distribution. All species of ''Nephroselmis'' are marine inhabitants except for ''Nephroselmis olivacea'' which is found strictly in freshwater environments.

The biofuel production potential of strains of ''Nephroselmis'' under certain treatments was examined in a research published in April 2022 concluding with - "isolated ''Nephroselmis'' sp. KGE2 is a candidate microalgal for biodiesel production using AMD as an iron source. The proposed process can be useful in overcoming the bottlenecks of microalgae cultivation. This can make the commercialization of biofuels economically feasible, and the products can be an alternative to fossil-derived commodities with zero carbon emissions."

A research published in January 2021 reveals new information about the diversity of algal phago-mixotrophy - “Our experimental results demonstrate the occurrence of bacterivory across all of the prasinophyte strains tested, spanning three prasinophyte groups: the Pyramimonadophyceae, the Nephroselmidophyceae, and the Mamiellophyceae”.

Life cycle

The life cycle that is found throughout the species of genus ''Nephroselmis'' is haplontic.

Cell division

Documented cell division of ''Nephroselmis astigmatica'' shows division by longitudinal binary fission.

Sexual reproduction

Observations of sexual reproduction in genus ''Nephroselmis'' are only known and were documented in the species ''Nephroselmis olivacea''. This species is also the only one in the genus that is known to be found in freshwater. The sexual mating mechanism is isogamous heterothallic, The two gamete types are plus and minus, they are morphologically similar but have different roles during the mating process. The minus gamete attaches to the substratum, The plus gamete attaches to the dorsal side of the minus gamete by the base of the flagella of the plus gamete.

Phylogeny and taxonomy

''Nephroselmis'' is monophyletic and repeatedly appears in phylogenetic analysis results as an early divergent of the core chlorophytes. Therefore the genus is phylogenetically important to understand the early evolution of Chlorophyta.

According to a 2021 paper ''Nephroselmis'' appeared as one of the earliest branching clades from the core chlorophytes. Yet the relationships between ''Nephroselmis'' to its sister groups is yet to be resolved. "The three ''prasinophyte'' lineages ''Nephroselmis'', ''Pycnococcus'', and ''Picocystis'' (abbreviated NPP) branched as the closest relatives to the core chlorophytes", "The relationships between the NPP lineages are not fully resolved".

Earlier paper published in 2011 showed similar results - "The class Nephroselmidophyceae is considered to be situated in the early radiation of Chlorophyta, but the strict phylogenetic position is uncertain".

Family Nephroselmidaceae was put under the order Pseudoscourfieldiales, that consisted of it and Pseudoscourfieldiaceae. The order Nephroselmidales was proposed in 2007- "The morphological evolution within the genus ''Nephroselmis'' is discussed and the order Nephroselmidales is proposed".

References

Chlorophyta genera
Nephrophyceae

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