Neoschizomers are

restriction enzymes A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class o ...

that recognize the same

nucleotide sequence A nucleic acid sequence is a succession of bases signified by a series of a set of five different letters that indicate the order of nucleotides forming alleles within a DNA (using GACT) or RNA (GACU) molecule. By convention, sequences are usua ...

as their prototype but cleave at a different site. In some special applications this is a very helpful feature. For example:Prototype MaeII A^CGT produces DNA fragments with a 2-base 5' extension Neoschizomer TaiI ACGT^ produces DNA fragments with a 4-base 3' extension Prototype ApaI GGGCC^C produces DNA fragments with a 4-base 3' extension Neoschizomer Bsp120I G^GGCCC produces DNA fragments with a 4-base 5' extension There are also other pairs of neoschizomers. Neoschizomers are a subset of

isoschizomer Isoschizomers are pairs of restriction enzymes specific to the same recognition sequence. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. The first enzyme discovered which recognizes a given sequence is known as th ...

References

* Rebase restriction enzyme database, http://rebase.neb.com/rebase/

References

See also