HOME

TheInfoList



Click Here for Items Related To - Nanovid microscopy
OR:
Nanovid microscopy on:   [Wikipedia]   [Google]   [Amazon]

{{Short description, Microscopic technique using colloidal gold Nanovid microscopy, from "nanometer video-enhanced microscopy", is a microscopic technique aimed at visualizing
colloidal gold Colloidal gold is a sol or colloidal suspension of nanoparticles of gold in a fluid, usually water. The colloid is usually either wine-red coloured (for spherical particles less than 100  nm) or blue/purple (for larger spherical particl ...
particles of 20–40 nm diameter (nanogold, immunogold) as dynamic markers at the light-microscopic level. The nanogold particles as such are smaller than the
diffraction Diffraction is defined as the interference or bending of waves around the corners of an obstacle or through an aperture into the region of geometrical shadow of the obstacle/aperture. The diffracting object or aperture effectively becomes a s ...
limit of
light Light or visible light is electromagnetic radiation that can be perceived by the human eye. Visible light is usually defined as having wavelengths in the range of 400–700 nanometres (nm), corresponding to frequencies of 750–420 tera ...
, but can be visualized by using video-enhanced differential interference contrast (VEDIC). The technique is based on the use of contrast enhancement by video techniques and digital
image processing An image is a visual representation of something. It can be two-dimensional, three-dimensional, or somehow otherwise feed into the visual system to convey information. An image can be an artifact, such as a photograph or other two-dimensiona ...
. Nanovid microscopy, by combining small colloidal gold probes with video-enhanced quantitative microscopy, allows studying the intracellular dynamics of specific
protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...
s in living
cells Cell most often refers to: * Cell (biology), the functional basic unit of life Cell may also refer to: Locations * Monastic cell, a small room, hut, or cave in which a religious recluse lives, alternatively the small precursor of a monastery w ...
.


See also

*
Microscopy Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of micr ...
*
Single-particle tracking Single-particle tracking (SPT) is the observation of the motion of individual particles within a medium. The coordinates time series, which can be either in two dimensions (''x'', ''y'') or in three dimensions (''x'', ''y'', ''z''), is referred to ...
*
Differential interference contrast microscopy Differential interference contrast (DIC) microscopy, also known as Nomarski interference contrast (NIC) or Nomarski microscopy, is an optical microscopy technique used to enhance the contrast in unstained, transparent samples. DIC works on the p ...
*
Microtubule Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to eukaryotic cells. Microtubules can be as long as 50 micrometres, as wide as 23 to 27  nm and have an inner diameter between 11 an ...


References

* De Mey, J., Moeremans, M., Geuens, G., Nuydens, R., De Brabander, M., High resolution light and electron microscopic localization of tubulin with the IGS (immuno-gold staining) method, Cell Biol. Int. Rep. 5, 889-899 (1981). * De Brabander M, Nuydens R, Geuens G, Moeremans M, De Mey J., The use of submicroscopic gold particles combined with video contrast enhancement as a simple molecular probe for the living cell, Cell Motil Cytoskeleton. 1986;6(2):105-13. * de Brabander, M. Nuydens, R. Ishihara, A. Holifield, B. Jacobson, K. and Geerts, H., Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy, J. Cell Biol., 112, 111-124 (1991). * Degelos SD, Wilson MP, Chandler JE., Nanovid microscopy for assessing sperm membrane changes induced by in vitro capacitating and acrosomal reacting procedures, J Androl. 1994 Sep-Oct;15(5):462-7. * Geerts H, De Brabander M, Nuydens R, Geuens S, Moeremans M, De Mey J, Hollenbeck P., Nanovid tracking: a new automatic method for the study of mobility in living cells based on colloidal gold and video microscopy, Biophys J. 1987 Nov;52(5):775-82. * Geerts H, de Brabander M, Nuydens R., Nanovid microscopy, Nature. 1991 Jun 27;351(6329):765-6. * Kusumi A, Sako Y, Yamamoto M., Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells, Biophys J. 1993 Nov;65(5):2021-40.


External links


Quantitative Microscopy
Cell imaging Microscopy Laboratory techniques