NS0 cells are a model cell line derived from the nonsecreting murine myeloma used in biomedical research and commercially in the production of therapeutic proteins. The cell line is a cholesterol-dependent cell line that was generated from a subline of NSI/1 which produced only the light chain but no heavy chain.

Cell line development

Development of murine neoplasms started with work with the

BALB/c BALB/c is an albino, laboratory-bred strain of the house mouse from which a number of common substrains are derived. Now over 200 generations from New York in 1920, BALB/c mice are distributed globally, and are among the most widely used inbred ...

mice to isolate the

IgG1 Immunoglobulin G (Ig G) is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG ...

secreting MOPC21 tumor. From this tumor, the P3K cells were isolated and developed into two cell lines, 289-16 and P3-X63. The 289-16 cell line secreted only light chain and no heavy chain and was renamed NSI/1. Clones from that cell line were isolated and a nonsecreting cell line was identified and was named NS0/1.

Uses in biotechnology

As myeloma cells, NSo cells are naturally antibody-producing suspension cells with a lymphoblast morphology. Gene amplification is typically performed using GS-transfected NS0 cells to select for producing cell lines. The GS-NS0 is a heterologous mammalian expression system that allows for the rapid expression of recombinant proteins. Several therapeutic antibody products are produced using the NS0 cell line including

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and

eculizumab Eculizumab, sold under the brand name Soliris among others, is a medication used to treat paroxysmal nocturnal hemoglobinuria (PNH), atypical hemolytic uremic syndrome (aHUS), generalized myasthenia gravis, and neuromyelitis optica. In people ...

References

External links

Cellosaurus entry for NS0
Rodent cell lines {{cell-biology-stub