Middlebrook 7H9 broth is a liquid growth medium specially used for culture of'' Mycobacterium'' species, notably ''

Mycobacterium tuberculosis ''Mycobacterium tuberculosis'' (M. tb) is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, ''M. tuberculosis'' has an unusual, waxy coating on its c ...

''.

Composition

* Ammonium sulfate * L-Glutamic acid * Sodium citrate * Pyridoxine * Biotin * Disodium phosphate * Monopotassium phosphate * Ferric ammonium citrate * Magnesium sulfate * Calcium chloride * Zinc sulfate * Copper Sulfate Middlebrook 7H9 broth supports the growth of mycobacterial species when supplemented with nutrients such as glycerol, oleic acid, albumin, and dextrose, except for ''

Mycobacterium bovis ''Mycobacterium bovis'' is a slow-growing (16- to 20-hour generation time) aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to ''Mycobacterium tuberculosis'', the bacterium which causes tube ...

'', which is inhibited by glycerol. Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks. Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests (arylsufatase and tellurite reduction), and maintenance of stock strains. Additionally, 7H9 broth is used as a medium in the

mycobacteria growth indicator tube Mycobacteria Growth Indicator Tube (MGIT) is intended for the culture, detection and recovery of mycobacteria. The MGIT Mycobacteria Growth Indicator Tube contains 7 mL of modified Middlebrook 7H9 Broth base. The complete medium, with OADC ...

References

External links

Middlebrook 7H9 Broths
{{DEFAULTSORT:Middlebrook 7h9 Broth Microbiological media

Composition

See also

References

External links