Murashige and Skoog medium (or ''MSO ''or '' MS0'' ''(MS-zero)'') is a

plant Plants are predominantly photosynthetic eukaryotes of the kingdom Plantae. Historically, the plant kingdom encompassed all living things that were not animals, and included algae and fungi; however, all current definitions of Plantae exclud ...

growth medium A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Differen ...

used in the laboratories for cultivation of plant

cell culture Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This te ...

. MS0 was invented by plant scientists

Toshio Murashige Toshio Murashige is a professor emeritus of University of California Riverside in plant biology. He is most widely known for his efforts in creating the plant tissue culture medium known as Murashige and Skoog medium Murashige and Skoog medium (o ...

and Folke K. Skoog in 1962 during Murashige's search for a new

plant growth regulator Plant hormone (or phytohormones) are signal molecules, produced within plants, that occur in extremely low concentrations. Plant hormones control all aspects of plant growth and development, from embryogenesis, the regulation of organ size, pat ...

. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example, MS0 contains no sucrose and MS20 contains 20 g/L sucrose. Along with its modifications, it is the most commonly used medium in

plant tissue culture Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known ...

experiments in the laboratory, but according to recent scientific findings MS medium should not be used as

nutrient A nutrient is a substance used by an organism to survive, grow, and reproduce. The requirement for dietary nutrient intake applies to animals, plants, fungi, and protists. Nutrients can be incorporated into cells for metabolic purposes or excret ...

solution in

deep water culture Deep water culture (DWC) is a hydroponic method of plant production by means of suspending the plant roots in a solution of nutrient-rich, oxygenated water. Also known as deep flow technique (DFT), floating raft technology (FRT), or raceway, this ...

. As Skoog's doctoral student, Murashige originally set out to find an as-yet undiscovered growth hormone present in

tobacco juice Tobacco water, tobacco juice, tobacco dust juice, or tobacco lime is a traditional organic insecticide used in domestic gardening. In ''The English Physician Enlarged'' of 1681, Nicholas Culpeper recommends tobacco juice to kill lice on children's h ...

. No such component was discovered; instead, analysis of juiced tobacco and ashed tobacco revealed higher

concentration In chemistry, concentration is the abundance of a constituent divided by the total volume of a mixture. Several types of mathematical description can be distinguished: '' mass concentration'', ''molar concentration'', ''number concentration'', an ...

s of specific

mineral In geology and mineralogy, a mineral or mineral species is, broadly speaking, a solid chemical compound with a fairly well-defined chemical composition and a specific crystal structure that occurs naturally in pure form.John P. Rafferty, ed. ( ...

s in plant tissues than were previously known. A series of experiments demonstrated that varying the levels of these

s enhanced

growth Growth may refer to: Biology * Auxology, the study of all aspects of human physical growth * Bacterial growth * Cell growth * Growth hormone, a peptide hormone that stimulates growth * Human development (biology) * Plant growth * Secondary growth ...

substantially over existing formulations. It was determined that

nitrogen Nitrogen is the chemical element with the symbol N and atomic number 7. Nitrogen is a nonmetal and the lightest member of group 15 of the periodic table, often called the pnictogens. It is a common element in the universe, estimated at se ...

in particular enhanced growth of tobacco in tissue culture.

Ingredients

Major
salts In chemistry, a salt is a chemical compound consisting of an ionic assembly of positively charged cations and negatively charged anions, which results in a compound with no net electric charge. A common example is table salt, with positively cha ...
(macronutrients) per litre

Ammonium nitrate Ammonium nitrate is a chemical compound with the chemical formula . It is a white crystalline salt consisting of ions of ammonium and nitrate. It is highly soluble in water and hygroscopic as a solid, although it does not form hydrates. It is ...

(NH₄NO₃) 1650 mg/l *

Calcium chloride Calcium chloride is an inorganic compound, a salt with the chemical formula . It is a white crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralising hydrochloric acid with calcium hydroxide. Ca ...

(CaCl₂ · 2H₂O) 440 mg/l *

Magnesium sulfate Magnesium sulfate or magnesium sulphate (in English-speaking countries other than the US) is a chemical compound, a salt with the formula , consisting of magnesium cations (20.19% by mass) and sulfate anions . It is a white crystalline solid, ...

(MgSO₄ · 7H₂O) 370 mg/l *

Monopotassium phosphate Monopotassium phosphate (MKP) (also, potassium dihydrogenphosphate, KDP, or monobasic potassium phosphate) is the inorganic compound with the formula KH2PO4. Together with dipotassium phosphate (K2HPO4.(H2O)x) it is often used as a fertilizer, f ...

(KH₂PO₄) 170 mg/l *

Potassium nitrate Potassium nitrate is a chemical compound with the chemical formula . This alkali metal nitrate salt is also known as Indian saltpetre (large deposits of which were historically mined in India). It is an ionic salt of potassium ions K+ and nitrat ...

(KNO₃) 1900 mg/l.

Minor salts (micronutrients) per litre

Boric acid Boric acid, more specifically orthoboric acid, is a compound of boron, oxygen, and hydrogen with formula . It may also be called hydrogen borate or boracic acid. It is usually encountered as colorless crystals or a white powder, that dissolves ...

(H₃BO₃) 6. 2 mg/l *

Cobalt chloride Cobalt chloride (cobalt paper) may refer to: * Cobalt(II) chloride (CoCl2) * Cobalt(III) chloride Cobalt(III) chloride or cobaltic chloride is an unstable and elusive compound of cobalt and chlorine with formula . In this compound, the cobalt at ...

(CoCl₂ · 6H₂O) 0.025 mg/l *

Ferrous sulfate Iron(II) sulfate (British English: iron(II) sulphate) or ferrous sulfate denotes a range of salts with the formula Fe SO4·''x''H2O. These compounds exist most commonly as the hepta hydrate (''x'' = 7) but several values for x are kno ...

(FeSO₄ · 7H₂O) 27.8 mg/l *

Manganese(II) sulfate Manganese(II) sulfate usually refers to the inorganic compound with the formula MnSO4·H2O. This pale pink deliquescent solid is a commercially significant manganese(II) salt. Approximately 260,000 tonnes of manganese(II) sulfate were produced w ...

(MnSO₄ · 4H₂O) 22.3 mg/l *

Potassium iodide Potassium iodide is a chemical compound, medication, and dietary supplement. It is a medication used for treating hyperthyroidism, in radiation emergencies, and for protecting the thyroid gland when certain types of radiopharmaceuticals are us ...

(KI) 0.83 mg/l *

Sodium molybdate Sodium molybdate, Na2MoO4, is useful as a source of molybdenum. This white, crystalline salt is often found as the dihydrate, Na2MoO4·2H2O. The molybdate(VI) anion is tetrahedral. Two sodium cations coordinate with every one anion.Braithwaite, E. ...

(Na₂MoO₄ · 2H₂O) 0.25 mg/l *

Zinc sulfate Zinc sulfate is an inorganic compound. It is used as a dietary supplement to treat zinc deficiency and to prevent the condition in those at high risk. Side effects of excess supplementation may include abdominal pain, vomiting, headache, and ti ...

(ZnSO₄·7H₂O) 8.6 mg/l * Ethylenediaminetetraacetic acid ferric sodium (FeNaEDTA) 36.70 mg/L *

Copper sulfate Copper sulfate may refer to: * Copper(II) sulfate, CuSO4, a common compound used as a fungicide and herbicide * Copper(I) sulfate Copper(I) sulfate, also known as cuprous sulfate, is an inorganic compound with the chemical formula Cu2 SO4. It ...

(CuSO₄ · 5H₂O) 0.025 mg/l

Vitamins and organic compounds per litre

Myo-Inositol Inositol, or more precisely ''myo''-inositol, is a carbocyclic sugar that is abundant in the brain and other mammalian tissues; it mediates cell signal transduction in response to a variety of hormones, neurotransmitters, and growth factors and ...

100 mg/l *

Nicotinic Acid Niacin, also known as nicotinic acid, is an organic compound and a form of vitamin B3, an essential human nutrient. It can be manufactured by plants and animals from the amino acid tryptophan. Niacin is obtained in the diet from a variet ...

0.5 mg/l *

Pyridoxine Pyridoxine, is a form of vitamin B6 found commonly in food and used as a dietary supplement. As a supplement it is used to treat and prevent pyridoxine deficiency, sideroblastic anaemia, pyridoxine-dependent epilepsy, certain metabolic disorde ...

HCl HCL may refer to: Science and medicine * Hairy cell leukemia, an uncommon and slowly progressing B cell leukemia * Harvard Cyclotron Laboratory, from 1961 to 2002, a proton accelerator used for research and development * Hollow-cathode lamp, a spe ...

0.5 mg/l *

Thiamine Thiamine, also known as thiamin and vitamin B1, is a vitamin, an essential micronutrient, that cannot be made in the body. It is found in food and commercially synthesized to be a dietary supplement or medication. Phosphorylated forms of thi ...

0.1 mg/l *

Glycine Glycine (symbol Gly or G; ) is an amino acid that has a single hydrogen atom as its side chain. It is the simplest stable amino acid (carbamic acid is unstable), with the chemical formula NH2‐ CH2‐ COOH. Glycine is one of the proteinogeni ...

2 mg/l *

Tryptone Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin. Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of '' E. coli'' and other microorganisms. It provides a so ...

1 g/l (optional) *

Indole Acetic Acid Indole-3-acetic acid (IAA, 3-IAA) is the most common naturally occurring plant hormone of the auxin class. It is the best known of the auxins, and has been the subject of extensive studies by plant physiologists. IAA is a derivative of indole, con ...

1-30 mg/l (optional) *

Kinetin Kinetin (/'kaɪnɪtɪn/) is a type of cytokinin, a class of plant hormone that promotes cell division. Kinetin was originally isolated by Carlos Miller and Skoog ''et al.'' as a compound from autoclaved herring sperm DNA that had cell division- ...

0.04-10 mg/l (optional)

References

*{{cite journal , title=A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures , journal=Physiologia Plantarum , year=1962 , volume=15 , issue=3 , doi=10.1111/j.1399-3054.1962.tb08052.x , pages= 473–497 , last1=Murashige , last2=Skoog , first1=T , first2=F, s2cid=84645704 Laboratory techniques Botany Cell culture media

Ingredients

Major salts In chemistry, a salt is a chemical compound consisting of an ionic assembly of positively charged cations and negatively charged anions, which results in a compound with no net electric charge. A common example is table salt, with positively cha ... (macronutrients) per litre

Minor salts (micronutrients) per litre

Vitamins and organic compounds per litre

See also

References

Major
salts In chemistry, a salt is a chemical compound consisting of an ionic assembly of positively charged cations and negatively charged anions, which results in a compound with no net electric charge. A common example is table salt, with positively cha ...
(macronutrients) per litre