Midichloria

"''Candidatus'' Midichloria" is a '' candidatus'' genus of Gram-negative, non-endospore-forming bacteria, with a bacillus shape around 0.45 µm in diameter and 1.2 µm in length. First described in 2004 with the temporary name IricES1, "''Candidatus'' Midichloria" species are symbionts of several species of hard ticks (''e.g.'', '' Ixodes ricinus'' and ''Ixodes uriae'' of the Ixodidae family). They live in the cells of the ovary of the females of this tick species. These bacteria have been observed in the mitochondria of the host cells, a trait that has never been described in any other symbiont of animals.

"''Candidatus'' Midichloria" bacteria seem to consume the mitochondria they parasitize, possibly using them as a source of energy and/or molecules to multiply. The interaction of these symbionts with their host is currently unknown, though the 100% prevalence in the females of the host tick suggests a mutualistic association. Although there is no evidence that even up to 20 bacteria residing within the host cell mitochondria produce deleterious effects in '' Rhipicephalus bursi'', the prevalence in ''R. bursi'' was estimated at 33% in females and 14% in males, suggesting that the relationship may be more complicated than previously thought. In ''I. ricinus'', between 10 to 20 bacteria are known to produce deleterious effects, leading to the conclusion that "''Candidatus'' Midichloria" consume mitochondria.

Species

Only one species, "''Candidatus'' Midichloria mitochondrii", is described in this genus. Molecular screenings, however, have detected the presence of related bacteria in other tick species, as well as in other blood-sucking arthropods, suggesting the possibility of horizontal transmission of these bacteria.

It was given its own family, " ''Candidatus'' Midichloriaceae", in the Rickettsiales. Some poorly studied candidate species belonging to this family may include '' Nicolleia massiliensis'' and the unclassified Montezuma strain.

Naming

The name of this bacterial genus, "''Candidatus'' Midichloria", is derived from the fictional midichlorians, a symbiotic, microscopic life form, which allow beings to communicate with the Force described in the fictional ''Star Wars'' universe.

Genome

The genome of "''Ca.'' M. mitochondrii" has been sequenced by an international scientific consortium formed by researchers at the University of Milan, the University of Sydney, the University of Valencia, the University of Pavia, and the University of Milan Bicocca.

The genome is 1.2 Mb, and it is, for most characteristics, very similar to the genomes of the other Rickettsiales, with two notable exceptions; the genome of ''M. mitochondrii'' contains the gene sets for the synthesis of the flagellum and of a cytochrome cbb3 oxidase.

"''Candidatus'' Midichloria" and the origin of mitochondria

The Rickettsiales are widely believed to be the closest relatives to mitochondria. Based on the fact that the "''Candidatus'' Midichloria" genes for the flagellum and for the ''cbb₃'' cytochrome oxidase were proven to be ancestral, the genes were inferred to have been present in the bacterium that established the symbiosis with the ancestor of the eukaryotic cell, which became the mitochondrion.

Hence, sequencing the genome of "''Ca.'' M. mitochondrii" allowed an improved reconstruction of the mitochondrion's hypothetical free-living ancestor: It was a motile bacterium able to survive in microaerophilic conditions. Both these characteristics may have played an important role in the beginning of the symbiosis between the eukaryotic cell and the mitochondrion.

References

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* Lo N, Beninati T, Sacchi L, Bandi C (2006b). An alpha-proteobacterium invades the mitochondria of the tick ''Ixodes ricinus''. In Insect Symbiosis II, edited by K. Bourtzis and T. Miller, ''CRC Press Boca Raton''.
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{{Taxonbar, from=Q6842280

Rickettsiales
Bacteria genera