Middlebrook 7H9 broth is a liquid growth medium specially used for culture of'' Mycobacterium'' species, notably ''
Mycobacterium tuberculosis''.
Composition
* Ammonium sulfate
* L-Glutamic acid
* Sodium citrate
* Pyridoxine
* Biotin
* Disodium phosphate
* Monopotassium phosphate
* Ferric ammonium citrate
* Magnesium sulfate
* Calcium chloride
* Zinc sulfate
* Copper Sulfate
Middlebrook 7H9 broth supports the growth of mycobacterial species when supplemented with nutrients such as glycerol, oleic acid, albumin, and dextrose, except for ''
Mycobacterium bovis
''Mycobacterium bovis'' is a slow-growing (16- to 20-hour generation time) aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to '' Mycobacterium tuberculosis'', the bacterium which causes tub ...
'', which is inhibited by glycerol. Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.
Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests (arylsufatase and tellurite reduction), and maintenance of stock strains.
Additionally, 7H9 broth is used as a medium in the
mycobacteria growth indicator tube.
See also
*
Lowenstein-Jensen medium
*
Middlebrook 7H10 agar
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of ''Mycobacterium'', notably ''Mycobacterium tuberculosis''. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly use ...
References
External links
Middlebrook 7H9 Broths
{{DEFAULTSORT:Middlebrook 7h9 Broth
Microbiological media