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Maturase K (matK) is a plant plastidial gene. The protein it encodes is an organelle intron maturase, a protein that splices
Maturase K (matK) is a plant plastidial gene. The protein it encodes is an organelle intron maturase, a protein that splices Group II intron
Group II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self- splicing) can occur under high-salt conditions ''in vitro''. However, ...
s. It is essential for ''in vivo'' splicing of Group II introns. Amongst other maturases, this protein retains only a well conserved domain X
Group II introns are a large class of self-catalytic ribozymes and mobile genetic elements found within the genes of all three domains of life. Ribozyme activity (e.g., self- splicing) can occur under high-salt conditions ''in vitro''. However, ...
and remnants of a reverse transcriptase
A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription. Reverse transcriptases are used by viruses such as HIV and hepatitis B to replicate their genomes, ...
domain.
Universal matK primers can be used for DNA barcoding
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "sequences"), an indiv ...
of angiosperms.
See also
* LtrA, an open reading frame found in the ''Lactococcus lactis'' group II introns LtrB. It is an intron-encoded protein, with three subdomains, one of which is a reverse-transcriptase/maturase.References
Plant genes {{gene-stub