The mallein test is a sensitive and specific clinical test for

glanders Glanders is a contagious zoonotic infectious disease that occurs primarily in horses, mules, and donkeys. It can be contracted by other animals, such as dogs, cats, pigs, goats, and humans. It is caused by infection with the bacterium ''Burkhold ...

, a common bacterial disease of

equids Equidae (sometimes known as the horse family) is the taxonomic family of horses and related animals, including the extant horses, asses, and zebras, and many other species known only from fossils. All extant species are in the genus '' Equus'', ...

(

horse The horse (''Equus ferus caballus'') is a domesticated, one-toed, hoofed mammal. It belongs to the taxonomic family Equidae and is one of two extant subspecies of ''Equus ferus''. The horse has evolved over the past 45 to 55 million yea ...

s, donkeys, mules). This test is an allergic hypersensitivity test used as a diagnosis for

. It is caused by a bacterium called ''

Burkholderia mallei ''Burkholderia mallei'' is a Gram-negative, bipolar, aerobic bacterium, a human and animal pathogen of genus ''Burkholderia'' causing glanders; the Latin name of this disease (''malleus'') gave its name to the species causing it. It is closely re ...

'', which is contagious for humans and other species. The occurrence of

must be reported to the

World Organisation for Animal Health The World Organisation for Animal Health (WOAH), formerly the (OIE), is an intergovernmental organization coordinating, supporting and promoting animal disease control. Mission and status The main objective of the WOAH is to control epizo ...

. Mallein, a protein fraction of ''B. mallei'', is usually injected by an eye-drop. If an animal is infected, the animal will show swelling in the eye from around 48 hours of injection and may be accompanied by secretion and

conjunctivitis Conjunctivitis, also known as pink eye, is inflammation of the outermost layer of the white part of the eye and the inner surface of the eyelid. It makes the eye appear pink or reddish. Pain, burning, scratchiness, or itchiness may occur. The ...

. Mallein is non toxic to normal animals.

History

Mallein was discovered in 1890. Before 1934, glanders was widespread. In the early 1900s, glanders was common in

Europe Europe is a large peninsula conventionally considered a continent in its own right because of its great physical size and the weight of its history and traditions. Europe is also considered a subcontinent of Eurasia and it is located entirel ...

, the

United States of America The United States of America (U.S.A. or USA), commonly known as the United States (U.S. or US) or America, is a country primarily located in North America. It consists of 50 states, a federal district, five major unincorporated territo ...

, and

Canada Canada is a country in North America. Its ten provinces and three territories extend from the Atlantic Ocean to the Pacific Ocean and northward into the Arctic Ocean, covering over , making it the world's second-largest country by tot ...

, and has been successfully eliminated by mass testing and the slaughter and destruction of campaigns. Glanders is still present in

Asia Asia (, ) is one of the world's most notable geographical regions, which is either considered a continent in its own right or a subcontinent of Eurasia, which shares the continental landmass of Afro-Eurasia with Africa. Asia covers an are ...

, the

Middle East The Middle East ( ar, الشرق الأوسط, ISO 233: ) is a geopolitical region commonly encompassing Arabia (including the Arabian Peninsula and Bahrain), Asia Minor (Asian part of Turkey except Hatay Province), East Thrace (Europ ...

and

South America South America is a continent entirely in the Western Hemisphere and mostly in the Southern Hemisphere, with a relatively small portion in the Northern Hemisphere at the northern tip of the continent. It can also be described as the sout ...

Limitations and benefits

Limitations

Although Mallein is the most commonly used form of testing for glanders, cross reactions were reported between ''

'' and '' Streptococcus equi'', which is a bacteria-caused contagious upper respiratory tract infection of

equines ''Equus'' , is a genus of mammals in the family (biology), family Equidae, which includes horses, donkeys, and zebras. Within the Equidae, ''Equus'' is the only recognized Extant taxon, extant genus, comprising seven living species. Like Equida ...

. This resulted in false-positive reactions.

Benefits

The specificity of Mallein tests and the efficiency and potency of it are connected to the "molecular weight of protein fractions, antigenic range, and

virulence Virulence is a pathogen's or microorganism's ability to cause damage to a host. In most, especially in animal systems, virulence refers to the degree of damage caused by a microbe to its host. The pathogenicity of an organism—its ability to ...

Test procedures and methods

* Preparation of crude mallein * Purification of malleo proteins *

Trichloroacetic acid Trichloroacetic acid (TCA; TCAA; also known as trichloroethanoic acid) is an analogue of acetic acid in which the three hydrogen atoms of the methyl group have all been replaced by chlorine atoms. Salts and esters of trichloroacetic acid are calle ...

(TCA) preparation *

Ammonium sulfate Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an inorganic salt with a number of commercial uses. The most common use is as a soil fertilizer. It contains 21% nitrogen a ...

precipitation * Gel filtration chromatography *

Sensitization Sensitization is a non-associative learning process in which repeated administration of a stimulus results in the progressive amplification of a response. Sensitization often is characterized by an enhancement of response to a whole class of sti ...

of animals *

Potency Potency may refer to: * Potency (pharmacology), a measure of the activity of a drug in a biological system * Virility * Cell potency, a measure of the differentiation potential of stem cells * In homeopathic dilutions, potency is a measure of how ...

test * Intradermopalpebral

References

External links

*{{Commonscatinline Horse diseases Veterinary diagnosis