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The Kato technique (also called the Kato–Katz technique) is a laboratory method for preparing human stool samples prior to searching for parasite eggs.


Indications

The Kato technique is now most commonly used for detecting
schistosome ''Schistosoma'' is a genus of trematodes, commonly known as blood flukes. They are parasitic flatworms responsible for a highly significant group of infections in humans termed ''schistosomiasis'', which is considered by the World Health Organi ...
eggs. It has in the past been used for other helminth eggs as well. It cannot be used to identify hookworm eggs because they collapse within 30 to 60 minutes of preparation using this method. One study of 299 subjects infected with '' Schistosoma mansoni'' found that the method had poor reproducibility and is therefore no longer recommended for primary health care settings: the problem may be that eggs of ''S. mansoni ''tend to clump together which means that even slides prepared from the same specimen may contain widely different egg counts. The other main argument against the Kato technique is that it is messy and therefore exposes technicians to an unnecessarily high risk of infection.


Method

The published methods vary in detail, but generally involves a standardized amount of sieved feces being examined under light microscopy, and subsequently get a standardized count of the amount of eggs therein, in terms of number of eggs per gram. It can possibly involve staining the fecal sample.


History

It was developed in 1954 by Japanese
medical laboratory scientist A medical laboratory scientist (MLS) or clinical laboratory scientist (CLS) or medical technologist (MT) performs diagnostic testing of blood and body fluids in clinical laboratories. The scope of a medical laboratory scientist's work begins wit ...
Dr. Katsuya Kato (1912–1991). The technique was modified for use in field studies in 1972 by a Brazilian team of researchers led by Brazilian parasitologist Naftale Katz (b.1940), and this modification was adopted by the
WHO Who or WHO may refer to: * Who (pronoun), an interrogative or relative pronoun * Who?, one of the Five Ws in journalism * World Health Organization Arts and entertainment Fictional characters * Who, a creature in the Dr. Seuss book '' Horton He ...
as a gold standard for multiple helminth infections.


See also

* Eggs per gram


References


Further reading

* {{cite journal , last1 = Glinz , first1 = D. , last2 = Silué , first2 = K.D. , last3 = Knopp , first3 = S. , last4 = Lohourignon , first4 = L.K. , last5 = Yao , first5 = K.P. , display-authors = etal , year = 2010 , title = Comparing Diagnostic Accuracy of Kato-Katz, Koga Agar Plate, Ether-Concentration, and FLOTAC for ''Schistosoma mansoni'' and Soil-Transmitted Helminths , journal = PLOS Neglected Tropical Diseases , volume = 4 , issue = 7, page = e754 , doi = 10.1371/journal.pntd.0000754 , pmid = 20651931 , pmc = 2907416 , doi-access = free Stool tests