KSL cells in
cell biology
Cell biology (also cellular biology or cytology) is a branch of biology that studies the structure, function, and behavior of cells. All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living and ...
are an early form of mouse/murine
hematopoietic
Haematopoiesis (, from Greek , 'blood' and 'to make'; also hematopoiesis in American English; sometimes also h(a)emopoiesis) is the formation of blood cellular components. All cellular blood components are derived from haematopoietic stem cells. ...
stem cell
In multicellular organisms, stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell. They are the earliest type o ...
s. Characteristics are Kit (+), Sca-1 (+) and Lin (-). HSCs
ematopoietic stem cellsin murine cultures show phenotypic markers as being CD34-, CD150+, and Flt3- for LTR
ong-term reconstitution These phenotypic markers are used when purifying hematopoietic stem cells from the many other differentiated cells in the bone marrow.
Kit (CD117) is the receptor of
Stem Cell Factor
Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoie ...
. Sca-1 is a murine hematopoietic stem cell
antigen. Lin is a series of lineage marker antigens that identify mature murine blood cells.
Hematopoitic stem cells are of interest because of their ability to self-renew and differentiate into every types of blood cell. Transplantation of these cells in the bone marrow can be used to treat leukemia and other diseases of the blood and immune system. Murine/mouse cells lines are used as a model to research treatments before they are tested on humans.
See also
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Cell biophysics Cell biophysics (or cellular biophysics) is a sub-field of biophysics that focuses on physical principles underlying cell function. Sub-areas of current interest include statistical models of intracellular signaling dynamics, intracellular transport ...
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Cell disruption
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Cell physiology
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Cellular adaptation
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Cellular microbiology
Cellular microbiology is a discipline that bridges microbiology and cell biology.
The term "cellular microbiology" was coined by the authors of the book of the same title published in 1996. Cooperation and mutual dependency between microbiology ...
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Outline of cell biology
Notes
University of Toronto, Faculty of Immunology,
J. Dick lab: Nat Immunol. 2010, 11:585; Science. 2011, 33 3:218
Rossi L, Challen GA, Sirin O, Lin KK, Goodell MA. Hematopoietic stem cell characterization and isolation. Methods Mol Biol. 2011;750:47-59.
doi: 10.1007/978-1-61779-145-1_3. PMID: 21618082; PMCID: PMC3621966.
Stem cells
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