James Ainslie may refer to:
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James Ainslie (cricketer) (1880–1953), Australian cricketer
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James Ainslie (pastoralist)
James Ainslie (1787–1844) was a Scottish soldier and shepherd, best known as the first overseer of the property known as Duntroon in the Australian Capital Territory. During his time on the Limestone Plains he is said to have had a relations ...
(1787–1844), Scottish pastoralist
{{hndis, Ainslie, James