Intelectins are
lectin
Lectins are carbohydrate-binding proteins that are highly specific for sugar groups that are part of other molecules, so cause agglutination of particular cells or precipitation of glycoconjugates and polysaccharides. Lectins have a role in rec ...
s (carbohydrate-binding proteins) expressed in humans and other
chordate
A chordate () is an animal of the phylum Chordata (). All chordates possess, at some point during their larval or adult stages, five synapomorphies, or primary physical characteristics, that distinguish them from all the other taxa. These fiv ...
s. Humans express two types of intelectins encoded by
ITLN1
Intelectin-1, also known as omentin or intestinal lactoferrin receptor, is an intelectin encoded in humans by the ''ITLN1'' gene. Intelectin-1 functions both as a receptor for bacterial arabinogalactans and for lactoferrin.
Having conserved ligan ...
and
ITLN2 gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...
s respectively.
Several intelectins bind microbe-specific carbohydrate residues. Therefore, intelectins have been proposed to function as immune lectins.
Even though intelectins contain fibrinogen-like domain found in the
ficolin
Ficolins are pattern recognition receptors that bind to acetyl groups present in the carbohydrates of bacterial surfaces and mediate activation of the lectin pathway of the complement cascade.
Structure
Ficolins (Fi+Col+Lin) are a group of oligo ...
s family of immune lectins, there is significant structural divergence.
Thus, intelectins may not function through the same lectin-complement pathway. Most intelectins are still poorly characterized and they may have diverse biological roles. Human intelectin-1 (hIntL-1) has also been shown to bind
lactoferrin
Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is a globular glycoprotein with a molecular mass of about 80 kDa that is widely represented in various secretory fluids, s ...
, but the functional consequence has yet to be elucidated. Additionally, hIntL-1 is a major component of asthmatic mucus and may be involved in insulin physiology as well.
Diversity
The first intelectin was discovered in ''
Xenopus laevis
The African clawed frog (''Xenopus laevis'', also known as the xenopus, African clawed toad, African claw-toed frog or the ''platanna'') is a species of African aquatic frog of the family Pipidae. Its name is derived from the three short claws ...
'' oocyte and is named XL35 or XCGL-1. ''X. laevis'' oocyte also contains a closely related XCGL-2. In addition, ''X. laevis'' embryos secrete Xenopus embryonic epidermal lectin into the environmental water, presumably to bind microbes. XSL-1 and XSL-2 are also expressed in ''X. laevis'' serum when stimulated with lipopolysaccharide. Two additional intestinal intelectins are discovered in ''X. laevis''
Human has two intelectins: hIntL-1 (omentin) and hIntL-2. Mouse also has two intelectins: mIntL-1 and mIntL-2.
Immune system
Several lines of evidence suggest that intelectins recognize microbes and may function as an innate immune defense protein. Tunicate intelectin is an opsonin for phagocytosis by hemocyte. Amphioxus intelectin has been shown to agglutinate bacteria. In zebrafish and rainbow trout, intelectin expression is stimulated upon microbial exposure. Mammals such as sheep and mice also upregulate intelectin expression upon parasitic infection.
Increase in intelectin expression upon microbial exposure support the hypothesis that intelectins play a role in the immune system.
Structure
Although intelectins require calcium ion for function, the sequences bear no resemblance to
C-type lectin
A C-type lectin (CLEC) is a type of carbohydrate-binding protein known as a lectin. The C-type designation is from their requirement for calcium for binding. Proteins that contain C-type lectin domains have a diverse range of functions including ...
s.
In addition, merely around 50 amino acids (the fibronogen-like domain) align with any known protein, specifically the
ficolin
Ficolins are pattern recognition receptors that bind to acetyl groups present in the carbohydrates of bacterial surfaces and mediate activation of the lectin pathway of the complement cascade.
Structure
Ficolins (Fi+Col+Lin) are a group of oligo ...
family.
The first structural details of an intelectin comes from the crystal structure of
selenomethionine
Selenomethionine (SeMet) is a naturally occurring amino acid. The L-selenomethionine enantiomer is the main form of selenium found in Brazil nuts, cereal grains, soybeans, and grassland legumes, while ''Se''-methylselenocysteine, or its γ-gluta ...
-labeled XEEL carbohydrate-recognition domain (Se-Met XEEL-CRD) solved by Se-
SAD.
XEEL-CRD was expressed and Se-Met-labeled in
High Five insect cells using a recombinant
baculovirus
''Baculoviridae'' is a family of viruses. Arthropod
Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropod ...
. The fibrinogen-like fold is conserved despite amino acid sequence divergence. However, extensive insertions are present in intelectin compared to ficolins, thus making intelectin a distinct lectin structural class.
The Se-Met XEEL-CRD structure then enables the structure solution by
molecular replacement
Molecular replacement (or MR) is a method of solving the phase problem in X-ray crystallography. MR relies upon the existence of a previously solved protein structure which is similar to our unknown structure from which the diffraction data is de ...
of D-glycerol 1-phosphate (GroP)-bound XEEL-CRD,
apo-human intelectin-1 (hIntL-1),
and galactofuranose-bound hIntL-1.
Each polypeptide chain of XEEL and hIntL-1 contains three bound calcium ions: two in the structural calcium site and one in the ligand binding site.
The amino acid residues in the structural calcium site are conserved among intelectins, thus it is likely that most, if not all, intelectins have two structural calcium ions.
In the ligand binding site of XEEL and hIntL-1, the exocyclic vicinal diol of the carbohydrate ligand directly coordinates to the calcium ion.
There are large variations in the ligand binding site residues among intelectin homologs suggesting that the intelectin family may have broad ligand specificities and biological functions.
As there is no intelectin numbering conventions in different organisms, one should not assume functional homology based on the intelectin number. For example, hIntL-1 has glutamic acid residues in the ligand binding site to coordinate a calcium ion, while zebrafish intelectin-1 are devoided of these acidic residues.
Zebrafish intelectin-2 ligand binding site residues are similar to those present in hIntL-1.
Oligomeric state
hIntL-1 is a disulfide-linked trimer as shown by non-reducing SDS-PAGE
and X-ray crystallography.
Despite lacking the intermolecular disulfide bonds, XEEL-CRD is trimeric in solution.
The N-terminal peptide of the full length XEEL is responsible for dimerizing the trimeric XEEL-CRD into a disulfide-linked hexameric full-length XEEL.
Therefore, the N-termini of intelectins are often responsible for forming disulfide-linked oligomer. In intelectin homologs where the N-terminal cysteines are absent, the CRD itself may still capable of forming non-covalent oligomer in solution.
References
Further reading
* for exhaustive ligand binding analysis of human intelectin-1 (hIntL-1). The article also reveals how hIntL-1 could discriminate between microbial and mammalian cells.
* for discussion on how the first intelectin structure (XEEL-CRD) was solved. In depth biophysical and evolutionary analyses of the intelectin family in the light of the available 3D structures also provide significant insights into this protein family not previously appreciated. The article serves as the most up-to-date review on the biochemistry of the intelectin family.
* for comprehensive genomics analysis of intelectins from various organisms.
{{refend
Lectins
Glycobiology
Immune system
Protein families
Protein structure