HIS-selective medium is a type
cell culture medium that lacks the
amino acid
Amino acids are organic compounds that contain both amino and carboxylic acid functional groups. Although hundreds of amino acids exist in nature, by far the most important are the alpha-amino acids, which comprise proteins. Only 22 alpha a ...
histidine. It can be used with bacteria reliant on the expression of a gene encoding proteins involved in histidine expression in order to survive.
[Joung, J. K., E. I. Ramm and C. O. Pabo (2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions." Proc Natl Acad Sci U S A 97(13): 7382-7.] Only bacteria expressing such genes (such as
hisB
The hisB gene, found in the enterobacteria (such as '' E. coli''), in ''Campylobacter jejuni'' and in '' Xylella''/''Xanthomonas'' encodes a protein involved in catalysis of two step in histidine biosynthesis (the sixth and eight step), namely the ...
in ''
Escherichia coli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esc ...
'' and
HIS3
The HIS3 gene, found in the ''Saccharomyces cerevisiae'' yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis. It is analogous to hisB in ''Escherichia coli''.
Exploi ...
in ''
Saccharomyces cerevisiae
''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have b ...
'') will survive on these media.
References
Cell culture media
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