High Five (BTI-Tn-5B1-4) is an insect

cell line An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cell ...

that originated from the ovarian cells of the

cabbage looper The cabbage looper (''Trichoplusia ni'') is a medium-sized moth in the family Noctuidae, a family commonly referred to as owlet moths. Its common name comes from its preferred host plants and distinctive crawling behavior. Cruciferous vegetable ...

, ''

Trichoplusia ni The cabbage looper (''Trichoplusia ni'') is a medium-sized moth in the family Noctuidae, a family commonly referred to as owlet moths. Its common name comes from its preferred host plants and distinctive crawling behavior. Cruciferous vegetab ...

.'' It was developed by the

Boyce Thompson Institute for Plant Research The Boyce Thompson Institute (previously: Boyce Thompson Institute for Plant Research) is an independent research institute devoted to using plant sciences to improve agriculture, protect the environment, and enhance human health. The Boyce Thomps ...

. High Five cells have become one of the most commonly used cell lines for recombinant protein expression using

baculovirus ''Baculoviridae'' is a family of viruses. Arthropod Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropod ...

transfection Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. It may also refer to other methods and cell types, although other terms are often preferred: "transformation" is typically used to desc ...

, and have been demonstrated to express more recombinant protein than other lepidopteran cell lines, such as

Sf9 SF9 ( ko, 에스에프나인 ; shortened from Sensational Feeling 9) is a South Korean boy band formed by FNC Entertainment and the company's first dance boy group. Consisting of nine members, the group debuted on October 5, 2016 with the relea ...

cells. The High Five cells have been used to produce the VLP-based HPV vaccine Cervarix. They can be grown in the absence of serum, and can be cultured in a loose attached state or in suspension High Five cells produce abundant

microRNA MicroRNA (miRNA) are small, single-stranded, non-coding RNA molecules containing 21 to 23 nucleotides. Found in plants, animals and some viruses, miRNAs are involved in RNA silencing and post-transcriptional regulation of gene expression. miRN ...

s (miRNAs),

small interfering RNA Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA at first non-coding RNA molecules, typically 20-24 (normally 21) base pairs in length, similar to miRNA, and operating wi ...

s (siRNAs), and PIWI-interacting RNAs (piRNAs), making it suitable to study all three types of small silencing RNAs. The High Five cell line, like other cell lines, has been found to host a non-pathogenic adventitious alphanodavirus. Researchers at the Boyce Thompson Institute were able to isolate virus-free sub-clones, named Tnao38 and Tnms42.

References

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External links

Cellosaurus entry for High FiveCellosaurus entry for virus-free sub-clone Tnao38Cellosaurus entry for virus-free sub-clone Tnms42High Five resource at the Boyce Thompson Institute
Insect cell lines