Hanks' salts is a collective group of
salts
In chemistry, a salt is a chemical compound consisting of an ionic assembly of positively charged cations and negatively charged anions, which results in a compound with no net electric charge. A common example is table salt, with positively cha ...
rich in
bicarbonate
In inorganic chemistry, bicarbonate (IUPAC-recommended nomenclature: hydrogencarbonate) is an intermediate form in the deprotonation of carbonic acid. It is a polyatomic anion with the chemical formula .
Bicarbonate serves a crucial biochemic ...
ions, formulated in 1940 by the microbiologist John H. Hanks. Typically, they are used as a
buffer
Buffer may refer to:
Science
* Buffer gas, an inert or nonflammable gas
* Buffer solution, a solution used to prevent changes in pH
* Buffering agent, the weak acid or base in a buffer solution
* Lysis buffer, in cell biology
* Metal ion buffer
* ...
system in
cell culture
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This te ...
media and aid in maintaining the optimum physiological
pH (roughly 7.0–7.4) for cellular growth. Due to their poorly
reactive
Reactive may refer to:
*Generally, capable of having a reaction (disambiguation)
*An adjective abbreviation denoting a bowling ball coverstock made of reactive resin
*Reactivity (chemistry)
*Reactive mind
*Reactive programming
See also
*Reactanc ...
nature and small concentration in solution, Hanks' salts are mainly used in media that are exposed to atmospheric conditions as opposed to
incubation. Performing the latter drastically exceeds the buffer capacity of Hanks' salts and may result in cell death.
The recipe according to AATBIO
Table 1. Required components
# Prepare 800 mL of distilled water in a suitable container.
# Add 8 g of NaCl to the solution.
# Add 400 mg of KCl to the solution.
# Add 140 mg of CaCl
2 to the solution.
# Add 100 mg of MgSO
4-7H
2O to the solution.
# Add 100 mg of MgCl
2-6H
2O to the solution.
# Add 60 mg of Na
2HPO
4-2H
2O to the solution.
# Add 60 mg of KH
2PO
4 to the solution.
# Add 1 g of D-Glucose (Dextrose) to the solution.
# Add 350 mg of NaHCO
3 to the solution.
# Add distilled water until volume is 1 L.
References
{{DEFAULTSORT:Hanks' Salts
Buffer solutions
Cell culture media