Gary J. Pielak (born July 17, 1955) is an American biological chemist who is known for developing quantitative techniques for measuring protein structure, stability, diffusion, and concentration in living cells, and under crowded conditions. Pielak is the Kenan Distinguished Professor of Chemistry, Biochemistry and Biophysics at the University of North Carolina at Chapel Hill School of Medicine, Lineberger Comprehensive Cancer Center, and Department of Chemistry. He is a Fellow of the Biophysical Society.

Education

Pielak earned a B.A. in chemistry, ''

cum laude Latin honors are a system of Latin phrases used in some colleges and universities to indicate the level of distinction with which an academic degree has been earned. The system is primarily used in the United States. It is also used in some Sou ...

,'' from Bradley University in 1977, and a Ph.D. in biochemistry 1983 from Washington State University under J. Ivan Legg. His dissertation was titled "Characterization of Arsanilazo & Sulfanilazo Proteins". He conducted postdoctoral work studying the functional role of certain amino acid residues on

cytochrome c The cytochrome complex, or cyt ''c'', is a small hemeprotein found loosely associated with the inner membrane of the mitochondrion. It belongs to the cytochrome c family of proteins and plays a major role in cell apoptosis. Cytochrome c is hig ...

electron transfer at the University of British Columbia with Nobel Prize-winner Michael Smith and at the University of Oxford with Robert J.P. Williams. He joined the Department of Chemistry at the University of North Carolina at Chapel Hill in 1989.

Research

Using in-cell nuclear magnetic resonance spectroscopy, a technique he helped develop, Pielak determined that the effects of in-cell crowding do not arise solely from the close-packed nature of the cytoplasm but rather that repulsive and attractive chemical interactions between cellular components determine the effects of macromolecular crowding. These interactions organize the inside of cells, controlling metabolism and

signaling In signal processing, a signal is a function that conveys information about a phenomenon. Any quantity that can vary over space or time can be used as a signal to share messages between observers. The ''IEEE Transactions on Signal Processing'' ...

. He and his collaborators have presented a quantitative model to explain crowding effects that is independent of crowder identity. For his work in this area, he received an NIH Director's Pioneer Award in 2006.

Selected honors

Gary Pielak has been on the Editorial Advisory Board of the journal '' Protein Science'', and serves on the Editorial Board of the journal ''Magnetic Resonance Letters'.'' He was an invited speaker at the 2017 Nobel Symposium on Protein Folding: ''From Mechanisms to Impact on Cells'', in Stockholm, Sweden. In 2023, Pielak received both the Johnston Teaching Excellence Award and the Faculty Award for Excellence in Doctoral Mentoring from the University of North Carolina at Chapel Hill. He also presented the McElvain Lecture at the University of Wisconsin, Madison that year. In 2024, he was an invited speaker at Protein Folding Dynamics

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in Galveston, Texas.

Selected publications

"Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs)" (2014). Francois-Xavier Theillet, Andres Binolfi, Tamara Frembgen-Kesner, Karan Hingorani, Mohona Sarkar, Ciara Kyne, Conggang Li, Peter B Crowley, Lila Gierasch, Gary J Pielak, Adrian H Elcock, Anne Gershenson, Philipp Selenko. ''Chemical Reviews'' 114 (13), 6661-6714. "FlgM gains structure in living cells" (2002). Matthew M Dedmon, Chetan N Patel, Gregory B Young, Gary J Pielak, ''Proceedings of the National Academy of Sciences'' 99 (20), 12681-12684. "Impact of Protein Denaturants and Stabilizers on Water Structure" (2004). Joseph D. Batchelor, Alina Olteanu, Ashutosh Tripathy, and Gary J. Pielak, ''J. Am. Chem. Soc.'' 2004, 126, 7, 1958–1961. "Macromolecular Crowding and Protein Stability" (2012). Yaqiang Wang, Mohona Sarkar, Austin E. Smith, Alexander S. Krois, and Gary J. Pielak, ''J. Am. Chem. Soc.'' 2012, 134, 40, 16614–16618.

References

{{DEFAULTSORT:Pielak, Gary American biochemists American biophysicists American chemists 1955 births Living people University of North Carolina School of Medicine faculty