The FluoProbes series of

fluorescent Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, ...

dyes were developed by

Interchim Interchim is a privately owned French company specialized in manufacturing and distribution of reagents, consumables and dedicated instruments for the R&D and industry laboratory in the fields of fine chemistry, chromatography and bio-analysis. ...

to improve performances of standard

fluorophore A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with se ...

s. They are designed for labeling biomolecules, cells, tissues or beads in advanced fluorescent detection techniques. * FluoProbes dyes are typically used to label proteins or nucleic acids (ultrafast -3 minutes- labeling of antibodies employs Lightning technology). Labeled products can be used for multiparameter detections, life time resolved fluorescence (TRF), polarisation anisotropy fluorescence, FRET, Quenching, FRAP. They are typically used in biotechnology and research applications as

fluorescence microscopy A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscop ...

, cell biology or

molecular biology Molecular biology is the branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including biomolecular synthesis, modification, mechanisms, and interactions. The study of chemical and physi ...

, as well as infrared imaging. Derivatives with

Amine In chemistry, amines (, ) are compounds and functional groups that contain a basic nitrogen atom with a lone pair. Amines are formally derivatives of ammonia (), wherein one or more hydrogen Hydrogen is the chemical element wi ...

and

Carboxyl In organic chemistry, a carboxylic acid is an organic acid that contains a carboxyl group () attached to an R-group. The general formula of a carboxylic acid is or , with R referring to the alkyl, alkenyl, aryl, or other group. Carboxylic ...

suit peptide and nucleic acid synthesis, while reactive ones (with succinimidyl,

maleimide Maleimide is a chemical compound with the formula H2C2(CO)2NH (see diagram). This unsaturated imide is an important building block in organic synthesis. The name is a contraction of maleic acid and imide, the -C(O)NHC(O)- functional group. Malei ...

and hydrazide) suit conjugation by conventional chemistry, while FluoProbes labeled antibodies and cellular probes (i.e.

Phalloidin Phalloidin belongs to a class of toxins called phallotoxins, which are found in the death cap mushroom ''(Amanita phalloides)''. It is a rigid bicyclic heptapeptide that is lethal after a few days when injected into the bloodstream. The major sy ...

) suit direct use in immunoassays or cell assays. * FluoProbes dyes that have comparable excitation and emission spectra to standard fluorophores such as

fluorescein Fluorescein is an organic compound and dye based on the xanthene tricyclic structural motif, formally belonging to triarylmethine dyes family. It is available as a dark orange/red powder slightly soluble in water and alcohol. It is widely used ...

rhodamine Rhodamine is a family of related dyes, a subset of the triarylmethane dyes. They are derivatives of xanthene. Important members of the rhodamine family are Rhodamine 6G, Rhodamine 123, and Rhodamine B. They are mainly used to dye paper and inks ...

s, cyanines Cy2/3/5/5.5/7, are claimed to solve limiting issues observed in some applications such as too high background, insufficient polarity,

photobleaching In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between ...

, insufficient brightness, or pH-sensitivity. I.e., FluoProbes488 reduces the background in Flow Cytometry and in slide microscopy allowing sharper and brighter images. FluoProbes 488, 547H and 647H are found more photostable that is taken to good account in applications using long illuminations periods (i.e. scanning such as in

confocal microscopy Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a s ...

),FluoProbes547H comparison in Confocal Microscopy
/ref> or for longer shelf-life of reagents (i.e. manufacturing diagnostics). * The

excitation Excitation, excite, exciting, or excitement may refer to: * Excitation (magnetic), provided with an electrical generator or alternator * Excite Ballpark, located in San Jose, California * Excite (web portal), web portal owned by IAC * Electron exc ...

and emission spectra of the FluoProbes series covers much of the

visible spectrum The visible spectrum is the portion of the electromagnetic spectrum that is visible to the human eye. Electromagnetic radiation in this range of wavelengths is called ''visible light'' or simply light. A typical human eye will respond to wa ...

up to the

infrared Infrared (IR), sometimes called infrared light, is electromagnetic radiation (EMR) with wavelengths longer than those of visible light. It is therefore invisible to the human eye. IR is generally understood to encompass wavelengths from around ...

region, matching the commonly used light sources and filters. Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes.

References

{{DEFAULTSORT:Fluoprobes Dyes Fluorescent dyes