Fim Switch

The ''fim'' switch in ''Escherichia coli'' is the mechanism by which the ''fim'' gene cluster, encoding Type I Pili, is transcriptionally controlled.

These pili are virulence factors involved in adhesion, especially important in uropathogenic ''Escherichia coli''. The gene undergoes phase variation mediated via two recombinases and is a model example of site specific inversion.

Structure and mechanism of phase variation

The operon consists of the promoter region ''fim'' S, the main constituent ''fim'' A, its gene product forming a rod like structure and ''fim'' H, coding for an adhesin at the tip, to name just a few important elements. The ''fim'' S region is flanked by 9bp repeats that are mirror images of each other. These mirror images serve as substrates for two ATP-dependent recombinases, ''fim'' B and ''fim'' E. These recombinases can invert the orientation of the ''fim'' S region and only one orientation allows for 3' to 5' transcription.

''fim'' B "flips" the promoter region both ways, from the "on" position to the "off" position and ''vice versa'', whereas ''fim'' E can only facilitate recombination from "on" to "off". This equilibrium, shifted towards maintaining the "off" position, due to higher ''fim'' E activity, serves as a mode of expressing pili only when adhesion is needed. Another level of transcriptional control in ''E. coli'' is mediated by the sensitivity of the recombinases to pH and osmolarity, further ensuring appropriate expression levels of type-I pili, given the stark differences in osmolarity inside and outside an animal's body. Type-I pili are expressed by many species of ''Enterobacteriaceae''. The transcriptional control can differ widely between species, in ''Salmonella typhimurium'', for example much influence is exerted by a leucine-responsive regulatory protein and there is no ''fim'' S element.

References

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Genetics
Escherichia coli
Gene expression
Virulence factors