A dry lab is a laboratory where the nature of the experiments does not involve significant risk. This is in contrast to a
wet lab A wet lab, or experimental lab, is a type of laboratory where it is necessary to handle various types of chemicals and potential "wet" hazards, so the room has to be carefully designed, constructed, and controlled to avoid spillage and contamination ...
where it is necessary to handle various types of chemicals and biological hazards. An example of a dry lab is one where computational or applied mathematical analyses are done on a computer-generated model to simulate a phenomenon in the physical realm. Examples of such phenomena include a molecule changing quantum states, the event horizon of a black hole or anything that otherwise might be impossible or too dangerous to observe under normal laboratory conditions. This term may also refer to a lab that uses primarily electronic equipment, for example, a robotics lab. A dry lab can also refer to a laboratory space for the storage of dry materials.
Dry labbing can also refer to supplying fictional (yet plausible) results in lieu of performing an assigned experiment, or carrying out a
systematic review.
''In silico'' chemistry
As computing power has grown exponentially this approach to research, often referred to as ''
in silico'' (as opposed to ''
in vitro
''In vitro'' (meaning in glass, or ''in the glass'') studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called " test-tube experiments", these studies in biology ...
'' and ''
in vivo
Studies that are ''in vivo'' (Latin for "within the living"; often not italicized in English) are those in which the effects of various biological entities are tested on whole, living organisms or cells, usually animals, including humans, and ...
''), has amassed more attention especially in the area of
bioinformatics. More specifically, within bioinformatics, is the study of proteins or
proteomics, which is the elucidation of their unknown structures and folding patterns. The general approach in the elucidation of protein structure has been to first purify a protein, crystallize it and then send X-rays through such a purified protein crystal to observe how these x-rays diffract into specific pattern—a process referred to as
X-ray crystallography
X-ray crystallography is the experimental science determining the atomic and molecular structure of a crystal, in which the crystalline structure causes a beam of incident X-rays to diffract into many specific directions. By measuring the angles ...
. However, many proteins, especially those embedded in cellular membranes, are nearly impossible to crystallize due to their hydrophobic nature. Although other techniques exists, such as
ramachandran plotting and
mass spectrometry, these alone generally do not lead to the full elucidation of protein structure or folding mechanisms.
Distributed computing
As a means of surpassing the limitations of these techniques, projects such as
Folding@home
Folding@home (FAH or F@h) is a volunteer computing project aimed to help scientists develop new therapeutics for a variety of diseases by the means of simulating protein dynamics. This includes the process of protein folding and the movements ...
and
Rosetta@home
Rosetta@home is a volunteer computing project researching protein structure prediction on the Berkeley Open Infrastructure for Network Computing (BOINC) platform, run by the Baker laboratory at the University of Washington. Rosetta@home aims ...
are aimed at resolving this problem using computational analysis, this means of resolving protein structure is referred to as
protein structure prediction
Protein structure prediction is the inference of the three-dimensional structure of a protein from its amino acid sequence—that is, the prediction of its secondary and tertiary structure from primary structure. Structure prediction is different ...
. Although many labs have a slightly different approach, the main concept is to find, from a myriad of protein conformations, which conformation has the lowest energy or, in the case of Folding@Home, to find relatively low energies of proteins that could cause the protein to misfold and aggregate other proteins to itself—like in the case of
sickle cell anemia
Sickle cell disease (SCD) is a group of blood disorders typically inherited from a person's parents. The most common type is known as sickle cell anaemia. It results in an abnormality in the oxygen-carrying protein haemoglobin found in red bl ...
. The general scheme in these projects is that a small number of computations are parsed to, or sent to be calculated on, a computer, generally a home computer, and then that computer analyzes the likelihood that a specific protein will take a certain shape or conformation based on the amount of energy required for that protein to stay in that shape, this way of processing data is what is generally referred to as
distributed computing
A distributed system is a system whose components are located on different networked computers, which communicate and coordinate their actions by passing messages to one another from any system. Distributed computing is a field of computer sci ...
. This analysis is done on an extraordinarily large number of different conformations, owing to the support of hundreds of thousands of home-based computers, with the goal of finding the conformation of lowest possible energy or set of conformations of lowest possible energy relative to any conformations that are just slightly different. Although doing so is quite difficult, one can, by observing the energy distribution of a large number of conformations, despite the almost infinite number of different protein conformations possible for any given protein (see
Levinthal Paradox
Levinthal's paradox is a thought experiment, also constituting a self-reference in the theory of protein folding. In 1969, Cyrus Levinthal noted that, because of the very large number of degrees of freedom in an unfolded polypeptide chain, the m ...
), with a reasonably large number of protein energy samplings, predict relatively closely what conformation, within a range of conformations, has the expected lowest energy using methods in
statistical inference. There are other factors such as salt concentration, pH, ambient temperature or
chaperonins, which are proteins that assist in the folding process of other proteins, that can greatly affect how a protein folds. However, if the given protein is shown to fold on its own, especially ''
in vitro
''In vitro'' (meaning in glass, or ''in the glass'') studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called " test-tube experiments", these studies in biology ...
'', these findings can be further supported. Once we can see how a protein folds then we can see how it works as a catalyst, or in intracellular communication, e.g. neuroreceptor-neurotransmitter interaction. How certain compounds may be used to enhance or prevent the function of these proteins and how an elucidated protein overall plays a role in disease can also be much better understood.
There are many other avenues of research in which the dry lab approach has been implemented. Other physical phenomena, such as sound, properties of newly discovered or hypothetical compounds and quantum mechanics models have recently received more attention in this area of approach.
As a method of deception
Dry labbing, as referring to the process of claiming results without actually doing a lab, is a notoriously shady practice that has been carried out through the ages. While dry labs are a huge problem today and cast question on modern research in some cases, their use dates back to
Archimedes, who claimed that heavier objects fall faster and lighter objects fall slower without actually carrying out the experiments himself. His error would not be fully corrected until the days of
Simon Stevin
Simon Stevin (; 1548–1620), sometimes called Stevinus, was a Flemish mathematician, scientist and music theorist. He made various contributions in many areas of science and engineering, both theoretical and practical. He also translated vario ...
and
Galileo.
See also
*
Computational chemistry
*
Computational science
*
Computer simulation
*
Computational physics
Computational physics is the study and implementation of numerical analysis to solve problems in physics for which a quantitative theory already exists. Historically, computational physics was the first application of modern computers in science, ...
*
In silico
*
Protein structure prediction
Protein structure prediction is the inference of the three-dimensional structure of a protein from its amino acid sequence—that is, the prediction of its secondary and tertiary structure from primary structure. Structure prediction is different ...
*
Wet lab A wet lab, or experimental lab, is a type of laboratory where it is necessary to handle various types of chemicals and potential "wet" hazards, so the room has to be carefully designed, constructed, and controlled to avoid spillage and contamination ...
References
{{Use dmy dates, date=November 2020
Bioinformatics
Laboratory types