Standard demineralizing solution
An aqueous 1 M solution of ethylenediaminetetraacetic acid (EDTA) is used adjusted to pH 10 with saturated sodium hydroxide solution. The cocoons are immersed in this solution for 72 h at 40 °C and gently stirred. During the process, the cocoons are kept under the surface of the solution by means of a plastic mesh, using a partial vacuum to encourage penetration of the solution. Afterwards, the cocoons are thoroughly washed with running tap water (three times 3 h) and subsequently reeled in tap water at room temperature using the standard method for ''Bombyx mori''. The method described here forms the basis of a patent application. (patent pending)Other solutions useful for demineralizing
Citric acid (present in lemon juice, orange juice, pineapple juice), formic acid, aluminum nitrate, and phosphate ions are all known to remove calcium oxalate by chelating calcium ions or by directly dissolving it.References
External links
{{YouTube, id=pn16vQwGeeI, title=Reeling Wild Silk: Gonometa postica Wild silk