Cryptobiosis or anabiosis is a metabolic state of life entered by an
organism
In biology, an organism () is any living system that functions as an individual entity. All organisms are composed of cells (cell theory). Organisms are classified by taxonomy into groups such as multicellular animals, plants, and ...
in response to adverse environmental conditions such as
desiccation
Desiccation () is the state of extreme dryness, or the process of extreme drying. A desiccant is a hygroscopic (attracts and holds water) substance that induces or sustains such a state in its local vicinity in a moderately sealed container.
...
,
freezing
Freezing is a phase transition where a liquid turns into a solid when its temperature is lowered below its freezing point. In accordance with the internationally established definition, freezing means the solidification phase change of a liquid o ...
, and
oxygen
Oxygen is the chemical element with the symbol O and atomic number 8. It is a member of the chalcogen group in the periodic table, a highly reactive nonmetal, and an oxidizing agent that readily forms oxides with most elements as wel ...
deficiency
A deficiency is generally a lack of something. It may also refer to:
*A deficient number, in mathematics, a number ''n'' for which ''σ''(''n'') < 2''n''
* . In the cryptobiotic state, all measurable metabolic processes stop, preventing
reproduction
Reproduction (or procreation or breeding) is the biological process by which new individual organisms – "offspring" – are produced from their "parent" or parents. Reproduction is a fundamental feature of all known life; each individual or ...
, development, and repair. When environmental conditions return to being hospitable, the organism will return to its
metabolic
Metabolism (, from el, μεταβολή ''metabolē'', "change") is the set of life-sustaining chemical reactions in organisms. The three main functions of metabolism are: the conversion of the energy in food to energy available to run cell ...
state of life as it was prior to the cryptobiosis.
Forms
Anhydrobiosis
Anhydrobiosis is the most studied form of cryptobiosis and occurs in situations of extreme
desiccation
Desiccation () is the state of extreme dryness, or the process of extreme drying. A desiccant is a hygroscopic (attracts and holds water) substance that induces or sustains such a state in its local vicinity in a moderately sealed container.
...
. The term ''anhydrobiosis'' derives from the Greek for "life without water" and is most commonly used for the
desiccation tolerance Desiccation tolerance refers to the ability of an organism to withstand or endure extreme dryness, or drought-like conditions. Plants and animals living in arid or periodically arid environments such as temporary streams or ponds may face the challe ...
observed in certain invertebrate animals such as
bdelloid rotifers
Bdelloidea (Greek ''βδέλλα'', ''bdella'', "leech") is a class of rotifers found in freshwater habitats all over the world. There are over 450 described species of bdelloid rotifers (or 'bdelloids'), distinguished from each other mainl ...
,
tardigrade
Tardigrades (), known colloquially as water bears or moss piglets, are a phylum of eight-legged segmented micro-animals. They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them Kleiner Wasserbär ...
s,
brine shrimp
''Artemia'' is a genus of aquatic crustaceans also known as brine shrimp. It is the only genus in the family Artemiidae. The first historical record of the existence of ''Artemia'' dates back to the first half of the 10th century AD from Urmia La ...
,
nematode
The nematodes ( or grc-gre, Νηματώδη; la, Nematoda) or roundworms constitute the phylum Nematoda (also called Nemathelminthes), with plant-Parasitism, parasitic nematodes also known as eelworms. They are a diverse animal phylum inhab ...
s, and at least one insect, a species of
chironomid
The Chironomidae (informally known as chironomids, nonbiting midges, or lake flies) comprise a family of nematoceran flies with a global distribution. They are closely related to the Ceratopogonidae, Simuliidae, and Thaumaleidae. Many species ...
(''
Polypedilum vanderplanki
''Polypedilum vanderplanki'' or the sleeping chironomid, is a dipteran in the family Chironomidae (non-biting midges). It occurs in the semi-arid regions of the African continent (e.g. northern Nigeria and Uganda). Its larvae are found in small t ...
''). However, other life forms exhibit desiccation tolerance. These include the
resurrection plant A resurrection plant is any poikilohydric plant that can survive extreme dehydration, even over months or years.
Examples include:
* ''Anastatica hierochuntica'', also known as the Rose of Jericho, a plant species native to deserts of North Africa ...
''
Craterostigma plantagineum'', the majority of plant seeds, and many microorganisms such as
bakers' yeast. Studies have shown that some anhydrobiotic organisms can survive for decades, even centuries, in the dry state.
Invertebrates undergoing anhydrobiosis often contract into a smaller shape and some proceed to form a
sugar
Sugar is the generic name for sweet-tasting, soluble carbohydrates, many of which are used in food. Simple sugars, also called monosaccharides, include glucose, fructose, and galactose. Compound sugars, also called disaccharides or double ...
called
trehalose
Trehalose (from Turkish '' tıgala'' – a sugar derived from insect cocoons + -ose) is a sugar consisting of two molecules of glucose. It is also known as mycose or tremalose. Some bacteria, fungi, plants and invertebrate animals synthesize it ...
. Desiccation tolerance in plants is associated with the production of another sugar,
sucrose
Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula .
For human consumption, sucrose is extracted and refined ...
. These sugars are thought to protect the organism from desiccation damage. In some creatures, such as bdelloid rotifers, no trehalose has been found, which has led scientists to propose other mechanisms of anhydrobiosis, possibly involving
intrinsically disordered proteins
In molecular biology, an intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure, typically in the absence of its macromolecular interaction partners, such as other proteins or RNA. IDPs rang ...
.
In 2011, ''
Caenorhabditis elegans
''Caenorhabditis elegans'' () is a free-living transparent nematode about 1 mm in length that lives in temperate soil environments. It is the type species of its genus. The name is a blend of the Greek ''caeno-'' (recent), ''rhabditis'' (ro ...
'', a nematode that is also one of the best-studied model organisms, was shown to undergo anhydrobiosis in the
dauer larva Dauer (German "''die Dauer''", English "the enduring", "the duration" in the meaning of "a length of time",) describes an alternative developmental stage of nematode worms, particularly rhabditids including '' Caenorhabditis elegans'', whereby the ...
stage. Further research taking advantage of genetic and biochemical tools available for this organism revealed that in addition to trehalose biosynthesis, a set of other functional pathways is involved in anhydrobiosis at the molecular level. These are mainly defense mechanisms against
reactive oxygen species
In chemistry, reactive oxygen species (ROS) are highly reactive chemicals formed from diatomic oxygen (). Examples of ROS include peroxides, superoxide, hydroxyl radical, singlet oxygen, and alpha-oxygen.
The reduction of molecular oxygen () p ...
and
xenobiotics
A xenobiotic is a chemical substance found within an organism that is not naturally produced or expected to be present within the organism. It can also cover substances that are present in much higher concentrations than are usual. Natural compo ...
, expression of
heat shock proteins
Heat shock proteins (HSP) are a family of proteins produced by cells in response to exposure to stressful conditions. They were first described in relation to heat shock, but are now known to also be expressed during other stresses including expo ...
and
intrinsically disordered proteins
In molecular biology, an intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure, typically in the absence of its macromolecular interaction partners, such as other proteins or RNA. IDPs rang ...
as well as biosynthesis of
polyunsaturated fatty acid
Polyunsaturated fatty acids (PUFAs) are fatty acids that contain more than one double bond in their backbone. This class includes many important compounds, such as essential fatty acids and those that give drying oils their characteristic proper ...
s and
polyamines
A polyamine is an organic compound having more than two amino groups. Alkyl polyamines occur naturally, but some are synthetic. Alkylpolyamines are colorless, hygroscopic, and water soluble. Near neutral pH, they exist as the ammonium derivatives. ...
. Some of them are conserved among anhydrobiotic plants and animals, suggesting that anhydrobiotic ability may depend on a set of common mechanisms. Understanding these mechanisms in detail might enable modification of non-anhydrobiotic cells, tissues, organs or even organisms so that they can be preserved in a dried state of
suspended animation
Suspended animation is the temporary (short- or long-term) slowing or stopping of biological function so that physiological capabilities are preserved. It may be either hypometabolic or ametabolic in nature. It may be induced by either endogen ...
over long time periods.
As of 2004, such an application of anhydrobiosis is being applied to
vaccine
A vaccine is a biological Dosage form, preparation that provides active acquired immunity to a particular infectious disease, infectious or cancer, malignant disease. The safety and effectiveness of vaccines has been widely studied and verifie ...
s. In vaccines, the process can produce a dry vaccine that reactivates once it is injected into the body. In theory, dry-vaccine technology could be used on any vaccine, including live vaccines such as the one for measles. It could also potentially be adapted to allow a vaccine's slow release, eliminating the need for boosters. This proposes to eliminate the need for refrigerating vaccines, thus making dry vaccines more widely available throughout the developing world where refrigeration, electricity, and proper storage are less accessible.
Based on similar principles, lyopreservation has been developed as a technique for preservation of biological samples at ambient temperatures. Lyopreservation is a
biomimetic
Biomimetics or biomimicry is the emulation of the models, systems, and elements of nature for the purpose of solving complex human problems. The terms "biomimetics" and "biomimicry" are derived from grc, βίος (''bios''), life, and μίμησ ...
strategy based on
anhydrobiosis
Cryptobiosis or anabiosis is a metabolic state of life entered by an organism in response to adverse environmental conditions such as desiccation, freezing, and oxygen deficiency. In the cryptobiotic state, all measurable metabolic processes sto ...
to preserve cells at ambient temperatures. It has been explored as an alternative technique for
cryopreservation
Cryo-preservation or cryo-conservation is a process where Organism, organisms, organelles, cell (biology), cells, Biological tissue, tissues, extracellular matrix, Organ (anatomy), organs, or any other biological constructs susceptible to damage ...
. The technique has the advantages of being able to preserve biological samples at ambient temperatures, without the need for refrigeration or use of cryogenic temperatures.
Anoxybiosis
In situations lacking
oxygen
Oxygen is the chemical element with the symbol O and atomic number 8. It is a member of the chalcogen group in the periodic table, a highly reactive nonmetal, and an oxidizing agent that readily forms oxides with most elements as wel ...
(a.k.a., anoxia), many cryptobionts (such as ''M. tardigradum'') take in water and become turgid and immobile, but can survive for prolonged periods of time. Some ectothermic vertebrates and some invertebrates, such as
brine shrimp
''Artemia'' is a genus of aquatic crustaceans also known as brine shrimp. It is the only genus in the family Artemiidae. The first historical record of the existence of ''Artemia'' dates back to the first half of the 10th century AD from Urmia La ...
s,
copepod
Copepods (; meaning "oar-feet") are a group of small crustaceans found in nearly every freshwater and saltwater habitat (ecology), habitat. Some species are planktonic (inhabiting sea waters), some are benthos, benthic (living on the ocean floor) ...
s, nematodes, and sponge
gemmule
Gemmules are internal buds found in sponges and are involved in asexual reproduction. It is an asexually reproduced mass of cells, that is capable of developing into a new organism i.e., an adult sponge.
Role in asexual reproduction
Asexual ...
s, are capable of surviving in a seemingly inactive state during anoxic conditions for months to decades.
Studies of the metabolic activity of these idling organisms during anoxia have been mostly inconclusive. This is because it is difficult to measure very small degrees of metabolic activity reliably enough to prove a cryptobiotic state rather than ordinary metabolic rate depression (MRD). Many experts are skeptical of the biological feasibility of anoxybiosis, as the organism is managing to prevent damage to its cellular structures from the environmental negative free energy, despite being both surrounded by plenty of water and thermal energy and without using any free energy of its own. However, there is evidence that the stress-induced protein p26 may act as a protein chaperone that requires no energy in cystic ''
Artemia franciscana
''Artemia franciscana'' is a species of brine shrimp endemic to the Americas but now widely introduced throughout the tropics and temperate zones worldwide.
Several late embryogenesis abundant proteins
Late embryogenesis abundant proteins (LEA ...
'' (
sea monkey
Sea-Monkeys is a marketing term for brine shrimp (''Artemia'') sold as novelty aquarium pets. Developed in the United States in 1957 by Harold von Braunhut, they are sold as eggs intended to be added to water, and almost always come bundled in a ...
) embryos, and most likely an extremely specialized and slow guanine polynucleotide pathway continues to provide metabolic free energy to the ''A. franciscana'' embryos during anoxic conditions. It seems that ''A. franciscana'' approaches but does not reach true anoxybiosis.
Chemobiosis
Chemobiosis is the cryptobiotic response to high levels of environmental toxins. It has been observed in
tardigrades
Tardigrades (), known colloquially as water bears or moss piglets, are a phylum of eight-legged segmented micro-animals. They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them Kleiner Wasserbär ...
.
Cryobiosis
Cryobiosis is a form of cryptobiosis that takes place in reaction to decreased
temperature
Temperature is a physical quantity that expresses quantitatively the perceptions of hotness and coldness. Temperature is measured with a thermometer.
Thermometers are calibrated in various temperature scales that historically have relied o ...
. Cryobiosis initiates when the water surrounding the organism's cells has been frozen. Stopping molecule mobility allows the organism to endure the freezing temperatures until more hospitable conditions return. Organisms capable of enduring these conditions typically feature molecules that facilitate freezing of water in preferential locations while also prohibiting the growth of large ice crystals that could otherwise damage cells. One such organism is the
lobster
Lobsters are a family (biology), family (Nephropidae, Synonym (taxonomy), synonym Homaridae) of marine crustaceans. They have long bodies with muscular tails and live in crevices or burrows on the sea floor. Three of their five pairs of legs ...
.
Osmobiosis
Osmobiosis is the least studied of all types of cryptobiosis. Osmobiosis occurs in response to increased
solute
In chemistry, a solution is a special type of homogeneous mixture composed of two or more substances. In such a mixture, a solute is a substance dissolved in another substance, known as a solvent. If the attractive forces between the solvent ...
concentration in the solution the organism lives in. Little is known for certain, other than that osmobiosis appears to involve a cessation of metabolism.
Examples
The brine shrimp ''Artemia salina'', which can be found in the
Makgadikgadi Pans
The Makgadikgadi Pan (Tswana pronunciation ), a salt pan situated in the middle of the dry savanna of north-eastern Botswana, is one of the largest salt flats in the world. The pan is all that remains of the formerly enormous Lake Makgadikg ...
in
Botswana
Botswana (, ), officially the Republic of Botswana ( tn, Lefatshe la Botswana, label=Setswana, ), is a landlocked country in Southern Africa. Botswana is topographically flat, with approximately 70 percent of its territory being the Kalahar ...
, survives over the dry season when the water of the pans evaporates, leaving a virtually desiccated lake bed.
The
tardigrade
Tardigrades (), known colloquially as water bears or moss piglets, are a phylum of eight-legged segmented micro-animals. They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them Kleiner Wasserbär ...
, or water bear, can undergo all five types of cryptobiosis. While in a cryptobiotic state, its
metabolism
Metabolism (, from el, μεταβολή ''metabolē'', "change") is the set of life-sustaining chemical reactions in organisms. The three main functions of metabolism are: the conversion of the energy in food to energy available to run cell ...
reduces to less than 0.01% of what is normal, and its water content can drop to 1% of normal. It can withstand extreme
temperature
Temperature is a physical quantity that expresses quantitatively the perceptions of hotness and coldness. Temperature is measured with a thermometer.
Thermometers are calibrated in various temperature scales that historically have relied o ...
,
radiation
In physics, radiation is the emission or transmission of energy in the form of waves or particles through space or through a material medium. This includes:
* ''electromagnetic radiation'', such as radio waves, microwaves, infrared, visi ...
, and
pressure
Pressure (symbol: ''p'' or ''P'') is the force applied perpendicular to the surface of an object per unit area over which that force is distributed. Gauge pressure (also spelled ''gage'' pressure)The preferred spelling varies by country and e ...
while in a cryptobiotic state.
Some
nematode
The nematodes ( or grc-gre, Νηματώδη; la, Nematoda) or roundworms constitute the phylum Nematoda (also called Nemathelminthes), with plant-Parasitism, parasitic nematodes also known as eelworms. They are a diverse animal phylum inhab ...
s and
rotifer
The rotifers (, from the Latin , "wheel", and , "bearing"), commonly called wheel animals or wheel animalcules, make up a phylum (Rotifera ) of microscopic and near-microscopic pseudocoelomate animals.
They were first described by Rev. John H ...
s can also undergo cryptobiosis.
See also
*
*
*
*
*
*
References
Further reading
* David A. Wharton, ''Life at the Limits: Organisms in Extreme Environments,'' Cambridge University Press, 2002, hardcover,
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