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Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to
Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion
Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen.
The commonly known types are:
:# Single diffusi ...
, but with the addition of an applied electrical field across the diffusion medium, usually an agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is ...
or polyacrylamide gel. The effect is rapid migration of the antibody and antigen out of their respective wells towards one another to form a line of precipitation, or a precipitin line, indicating binding.
See also
*Electrophoresis
Electrophoresis, from Ancient Greek ἤλεκτρον (ḗlektron, "amber") and φόρησις (phórēsis, "the act of bearing"), is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric fie ...
* Immunoelectrophoresis
References
*External links
* * https://web.archive.org/web/20070613005107/http://www.lib.mcg.edu/edu/esimmuno/ch4/electro.htm Immunologic tests Blood tests {{immunology-stub