Cofactor of BRCA1, also known as COBRA1, is a human
gene
In biology, the word gene (from , ; "... Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a b ...
that encodes NELF-B.
Function
NELF-B is a subunit of
negative elongation factor (NELF), which also includes NELF-A (
WHSC2
Negative elongation factor A is a protein that in humans is encoded by the ''WHSC2'' gene.
Function
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with ...
; MIM 606026), either NELF-C or NELF-D (TH1L; MIM 605297), and NELF-E (
RDBP
Negative elongation factor E is a protein that in humans is encoded by the ''RDBP'' gene.
Function
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elo ...
; MIM 154040). NELF acts with DRB sensitivity-inducing factor (
DSIF In gene expression, DSIF ( DRB Sensitivity Inducing Factor) is a protein that can either negatively or positively affect transcription by RNA polymerase II (Pol II). In one case of negative regulation, it can interact with negative elongation facto ...
), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of
RNA polymerase II
RNA polymerase II (RNAP II and Pol II) is a multiprotein complex that transcribes DNA into precursors of messenger RNA (mRNA) and most small nuclear RNA (snRNA) and microRNA. It is one of the three RNAP enzymes found in the nucleus of eukaryo ...
(see MIM 180660).
COBRA1 was initially identified in a yeast two-hybrid screen using the BRCT1 domain of
BRCA1
Breast cancer type 1 susceptibility protein is a protein that in humans is encoded by the ''BRCA1'' () gene. Orthologs are common in other vertebrate species, whereas invertebrate genomes may encode a more distantly related gene. ''BRCA1'' is a ...
as bait.
Interactions
Cofactor of BRCA1 has been shown to
interact
Advocates for Informed Choice, dba interACT or interACT Advocates for Intersex Youth, is a 501(c)(3) nonprofit organization using innovative strategies to advocate for the legal and human rights of children with intersex traits. The organizati ...
with:
*
BRCA1
Breast cancer type 1 susceptibility protein is a protein that in humans is encoded by the ''BRCA1'' () gene. Orthologs are common in other vertebrate species, whereas invertebrate genomes may encode a more distantly related gene. ''BRCA1'' is a ...
*
C-Fos,
*
C-jun,
*
Estrogen receptor alpha
Estrogen receptor alpha (ERĪ±), also known as NR3A1 (nuclear receptor subfamily 3, group A, member 1), is one of two main types of estrogen receptor, a nuclear receptor (mainly found as a chromatin-binding protein)
that is activated by the sex ...
,
*
RDBP
Negative elongation factor E is a protein that in humans is encoded by the ''RDBP'' gene.
Function
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elo ...
,
and
*
TH1L.
References
Further reading
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
{{refend