History
In the late 1700s, Germany experienced a number of outbreaks of an illness that seemed connected to eating certain sausages. In 1817, the German neurologist Justinus Kerner detected rod-shaped cells in his investigations into this so-called sausage poisoning. In 1897, the Belgian biology professorTaxonomy
As of October 2022, there are 164 validly published species in ''Clostridium''.Page ''Genus: Clostridium'' on The genus, as traditionally defined, contains many organisms not closely related to its type species. The issue was originally illustrated in full detail by a rRNA phylogeny from Collins 1994, which split the traditional genus (now corresponding to a large slice of Clostridia) into twenty clusters, with cluster ''I'' containing the type species and its close relatives. Over the years, this has resulted in many new genera being split out, with the ultimate goal of constraining ''Clostridium'' to cluster ''I''. "Clostridium" cluster ''XIVa'' and "Clostridium" cluster ''IV'' efficiently ferment plant polysaccharide composing dietary fiber, making them important and abundant taxa in the rumen and the human large intestine. As mentioned before, these clusters are not part of current ''Clostridium'', and use of these terms should be avoided due to ambiguous or inconsistent usage.Biochemistry
Species of ''Clostridium'' are obligate anaerobe and capable of producing endospores. They generally stain Gram-positive bacteria, gram-positive, but as well as '' Bacillus'', are often described as Gram-variable, because they show an increasing number of gram-negative cells as the culture ages. The normal, reproducing cells of ''Clostridium'', called the vegetative form, are Bacillus (shape), rod-shaped, which gives them their name, from the Greek (language), Greek κλωστήρ or spindle. ''Clostridium'' Endospores have a distinct bowling pin or bottle shape, distinguishing them from other bacterial endospores, which are usually ovoid in shape. The Schaeffer-Fulton stain (0.5% malachite green in water) can be used to distinguish endospores of ''Bacillus'' and ''Clostridium'' from other microorganisms. ''Clostridium'' can be differentiated from the also Endspore forming genus ''Bacillus'' by its obligate anaerobic growth, the shape of endospores and the lack of catalase. Species of ''Desulfotomaculum'' form similar endospores and can be distinguished by their requirement for sulfur. Glycolysis andBiology and pathogenesis
''Clostridium'' species are readily found inhabiting soils and intestinal tracts. ''Clostridium'' species are also a normal List of microbiota species of the lower reproductive tract of women, inhabitant of the healthy lower reproductive tract of females. The main species responsible for disease in humans are: * ''Clostridium botulinum'' can produce botulinum toxin in food or wounds and can cause botulism. This same toxin is known as Botox and is used in cosmetic surgery to paralyze facial muscles to reduce the signs of aging; it also has numerous other therapeutic uses. * ''Clostridium perfringens'' causes a wide range of symptoms, from Foodborne illness, food poisoning to cellulitis, fasciitis, necrotic enteritis and gas gangrene. * ''Clostridium tetani'' causes tetanus. Several more pathogens were previously described in ''Clostridium'', but have been moved to other genera with additional research. * ''Clostridioides difficile, Clostridium difficile'', now placed in ''Clostridioides''. * ''Hathewaya_histolytica, Clostridium histolyticum'', now placed in ''Hathewaya''. * ''Paeniclostridium sordellii, Clostridium sordellii'', now placed in ''Paeniclostridium'', can cause a fatal infection in exceptionally rare cases after medical abortions.Treatment
In general, the treatment of clostridial infection is high-dose penicillin G, to which the organism has remained susceptible. ''Clostridium welchii'' and ''Clostridium tetani'' respond to Sulfonamide (medicine), sulfonamides. Clostridia are also susceptible to tetracyclines, carbapenems (imipenem), metronidazole, vancomycin, and chloramphenicol. The vegetative cells of clostridia are heat-labile and are killed by short heating at temperatures above 72–75 °C. The thermal destruction of ''Clostridium'' spores requires higher temperatures (above 121.1 °C, for example in an autoclave) and longer cooking times (20 min, with a few exceptional cases of > 50 min recorded in the literature). ''Clostridia'' and ''Bacilli'' are quite radiation-resistant, requiring doses of about 30 kGy, which is a serious obstacle to the development of shelf-stable irradiated foods for general use in the retail market. The addition of lysozyme, nitrate, nitrite and propionic acid salts inhibits clostridia in various foods. Fructooligosaccharides (fructans) such as inulin, occurring in relatively large amounts in a number of foods such as chicory, garlic, onion, leek, artichoke, and asparagus, have a prebiotic (nutrition), prebiotic or Bifidus factor, bifidogenic effect, selectively promoting the growth and metabolism of beneficial bacteria in the Large intestine, colon, such as bifidobacteria and lactobacilli, while inhibiting harmful ones, such as clostridia, fusobacteria, and bacteroides.Use
* ''Clostridium thermocellum'' can use lignocellulosic waste and generate ethanol, thus making it a possible candidate for use in production of ethanol fuel. It also has no oxygen requirement and is Thermophile, thermophilic, which reduces cooling cost. * ''Clostridium acetobutylicum'' was first used by Chaim Weizmann to produce acetone and biobutanol from starch in 1916 for the production of cordite (smokeless gunpowder). * ''Clostridium botulinum'' produces a potentially lethal neurotoxin used in a diluted form in the drug Botox, which is carefully injected to nerves in the face, which prevents the movement of the expressive muscles of the forehead, to delay the wrinkling effect of aging. It is also used to treat spasmodic torticollis and provides relief for around 12 to 16 weeks. * ''Clostridium butyricum'' MIYAIRI 588 strain is marketed in Japan, Korea, and China for ''Clostridium difficile (bacteria), Clostridium difficile'' prophylaxis due to its reported ability to interfere with the growth of the latter. * ''Clostridium histolyticum'' has been used as a source of the enzyme collagenase, which degrades animal tissue. Clostridium species excrete collagenase to eat through tissue and, thus, help the pathogen spread throughout the body. The medical profession uses collagenase for the same reason in the débridement of infected wounds. Hyaluronidase, deoxyribonuclease, lecithinase, leukocidin, protease, lipase, and hemolysin are also produced by some clostridia that cause gas gangrene. * ''Clostridium ljungdahlii'', recently discovered in commercial chicken wastes, can produce ethanol from single-carbon sources including synthesis gas, a mixture of carbon monoxide and hydrogen, that can be generated from the partial combustion of either fossil fuels or biomass. * ''Clostridium butyricum'' converts glycerol to 1,3-propanediol. * Genes from ''Clostridium thermocellum'' have been inserted into transgenic mice to allow the production of endoglucanase. The experiment was intended to learn more about how the digestive capacity of monogastric animals could be improved. * Nonpathogenic strains of ''Clostridium'' may help in the treatment of diseases such as cancer. Research shows that ''Clostridium'' can selectively target cancer cells. Some strains can enter and replicate within solid tumors. ''Clostridium'' could, therefore, be used to deliver therapeutic proteins to tumours. This use of ''Clostridium'' has been demonstrated in a variety of preclinical models. * Mixtures of ''Clostridium'' species, such as ''Clostridium beijerinckii'', ''Clostridium butyricum'', and species from other genera have been shown to produce biohydrogen from yeast waste.References
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