Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of
alkaline phosphatase
The enzyme alkaline phosphatase (EC 3.1.3.1, alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum), systematic ...
that catalyzes the removal of
phosphate groups from the 5' end of DNA strands and
phosphomonoesters Phosphomonoesters (or phosphoric esters) are chemical compounds containing one ester bond and a phosphate group.
In biology, phosphomonoesters are needed as the building blocks for the synthesis of Phospholipid cellular membranes, especially those ...
from RNA.
This enzyme is frequently used in DNA
sub-cloning
In molecular biology, subcloning is a technique used to move a particular DNA sequence from a ''parent vector'' to a ''destination vector''.
Subcloning is not to be confused with molecular cloning, a related technique.
Procedure
Restriction e ...
, as DNA fragments that lack the 5' phosphate groups cannot
ligate.
This prevents recircularization of the linearized DNA
vector and improves the yield of the vector containing the appropriate insert.
References
Enzymes
Genetics techniques
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