BOD bottle
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BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 
°C The degree Celsius is the unit of temperature on the Celsius scale (originally known as the centigrade scale outside Sweden), one of two temperature scales used in the International System of Units (SI), the other being the Kelvin scale. The ...
or 68  °F of temperature.


Structure

The bottle is normally designed to have a special shoulder radius to push out all air from the inside of the bottle when a sample solution is being filled. According to Method 5210 in ''Standard Methods for the Examination of Water and Wastewater'', the BOD bottle should include a ground-glass stopper and a flared mouth which form a water seal preventing the air from the outside of the bottle coming in. Method 5210 also recommends to use a paper, a
foil Foil may refer to: Materials * Foil (metal), a quite thin sheet of metal, usually manufactured with a rolling mill machine * Metal leaf, a very thin sheet of decorative metal * Aluminium foil, a type of wrapping for food * Tin foil, metal foil ...
or a plastic cup to cap over the mouth of the bottle reducing the
evaporation Evaporation is a type of vaporization that occurs on the surface of a liquid as it changes into the gas phase. High concentration of the evaporating substance in the surrounding gas significantly slows down evaporation, such as when humidi ...
during the incubation. Generally, the side of the BOD bottle is permanently screened with white writing area, and is printed with a specific number; both for the aid of the sample identification. ;Stopper There are two kinds of stopper: the Glass Pennyhead and the Glass Robotic stopper. ;Sizes There are many BOD bottle sizes. The dose of the
mixture In chemistry, a mixture is a material made up of two or more different chemical substances which are not chemically bonded. A mixture is the physical combination of two or more substances in which the identities are retained and are mixed in the ...
of the
solution Solution may refer to: * Solution (chemistry), a mixture where one substance is dissolved in another * Solution (equation), in mathematics ** Numerical solution, in numerical analysis, approximate solutions within specified error bounds * Soluti ...
(
nutrient A nutrient is a substance used by an organism to survive, grow, and reproduce. The requirement for dietary nutrient intake applies to animals, plants, fungi, and protists. Nutrients can be incorporated into cells for metabolic purposes or excret ...
,
mineral In geology and mineralogy, a mineral or mineral species is, broadly speaking, a solid chemical compound with a fairly well-defined chemical composition and a specific crystal structure that occurs naturally in pure form.John P. Rafferty, ed. ( ...
and
buffer solution A buffer solution (more precisely, pH buffer or hydrogen ion buffer) is an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa. Its pH changes very little when a small amount of strong acid or base is ...
) is related to the size of the bottle. For the Standard Methods 5210, the BOD bottle “having 60 mL or greater capacity (300-mL)” is mentioned as one of the apparatus for the BOD test. A 60 mL BOD bottle is available and listed as "often convenient" by
EPA The Environmental Protection Agency (EPA) is an independent executive agency of the United States federal government tasked with environmental protection matters. President Richard Nixon proposed the establishment of EPA on July 9, 1970; it be ...
(Environmental Protection Agency) Method 405.1. However, EPA Method 405.1 was written in 1974 and is no longer an EPA-approved method per 40CFR Part 136.


Materials

Glass Glass is a non-crystalline, often transparent, amorphous solid that has widespread practical, technological, and decorative use in, for example, window panes, tableware, and optics. Glass is most often formed by rapid cooling (quenching) of ...
is a material being specified in the Standard Methods 5210 of the BOD5 test. The glass bottles are manufactured from Type 1
borosilicate glass Borosilicate glass is a type of glass with silica and boron trioxide as the main glass-forming constituents. Borosilicate glasses are known for having very low coefficients of thermal expansion (≈3 × 10−6 K−1 at 20 °C), ma ...
. ;A black BOD bottle A black BOD bottle is coated with PVC plastic that blocks
visible light Light or visible light is electromagnetic radiation that can be perceived by the human eye. Visible light is usually defined as having wavelengths in the range of 400–700 nanometres (nm), corresponding to frequencies of 750–420 te ...
. Black bottles are used in marine
photosynthesis Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that, through cellular respiration, can later be released to fuel the organism's activities. Some of this chemical energy is stored i ...
projects which needs to compare oxygen levels in light and dark conditions. ;Disposable BOD Bottle or Carbonaceous Biochemical Oxygen Demand (CBOD) Bottle It is a carbon-coated polyethylene terephthalate (PET) bottle that is solely manufactured by Environmental Express in Charleston, SC. The bottle is lightweight, unbreakable, and recyclable. Since the bottle is designed for single-use, it eliminates any potential for cross-contamination between samples. The bottle does not require any resources nor energy for cleaning and rinsing as it is disposable. CBOD bottle is also claimed to be cheaper, and cause less
contamination Contamination is the presence of a constituent, impurity, or some other undesirable element that spoils, corrupts, infects, makes unfit, or makes inferior a material, physical body, natural environment, workplace, etc. Types of contamination W ...
in the sample solution than the conventional- BOD bottle.


References

{{Reflist Measuring instruments Liquid containers