Bis-tris methane, also known as BIS-TRIS or BTM, is a
buffering agent used in
biochemistry. Bis-tris methane is an organic tertiary
amine with labile protons having a
pKa
PKA may refer to:
* Professionally known as:
** Pen name
** Stage persona
* p''K''a, the symbol for the acid dissociation constant at logarithmic scale
* Protein kinase A, a class of cAMP-dependent enzymes
* Pi Kappa Alpha, the North-American so ...
of 6.46 at 25 °C. It is an effective buffer between the
pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.
See also
*
Bis-tris propane
Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, also known as BTP, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering ran ...
*
Tris
*
Tricine
References
Polyols
Amines
Buffer solutions
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