Alcian yellow is an azo dye with the formula (CH₃ CH₃N)₂CSsub>2C₆H₂SNCC₆H₄)₂N₂. Its structure consists of a largely conjugated array of

benzene Benzene is an organic chemical compound with the molecular formula C6H6. The benzene molecule is composed of six carbon atoms joined in a planar ring with one hydrogen atom attached to each. Because it contains only carbon and hydrogen atoms ...

benzothiazole Benzothiazole is an aromatic heterocyclic compound with the chemical formula . It is colorless, slightly viscous liquid. Although the parent compound, benzothiazole is not widely used, many of its derivatives are found in commercial products or ...

and

isothiouronium In organic chemistry, isothiouronium is a functional group with the formula SC(NH2)2sup>+ (R = alkyl, aryl) and is the acid salt of isothiourea. The H centres can also be replaced by alkyl and aryl. Structurally, these cations resemble guanid ...

units.

Histological Use

Alcian yellow is used in

histology Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures vi ...

in conjunction with

toluidine blue Toluidine blue, also known as TBO or tolonium chloride ( INN) is a blue cationic (basic) dye used in histology (as the toluidine blue stain) and sometimes clinically. Test for lignin Toluidine blue solution is used in testing for lignin, a comp ...

to help visualize ''H. pylori''. The resulting structures are stained as follows: –

Mucin Mucins () are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals. Mucins' key characteristic is their ability to form gels; therefore they are a key component in most ...

: yellow – H. pylori: blue – Other cellular components/background: shades of blue

References

{{Reflist Azo dyes Benzothiazoles

Histological Use

See also

References