Adenylyl-sulfate reductase () is an

enzyme Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. A ...

that

catalyzes Catalysis () is the process of increasing the rate of a chemical reaction by adding a substance known as a catalyst (). Catalysts are not consumed in the reaction and remain unchanged after it. If the reaction is rapid and the catalyst recyc ...

the

chemical reaction A chemical reaction is a process that leads to the IUPAC nomenclature for organic transformations, chemical transformation of one set of chemical substances to another. Classically, chemical reactions encompass changes that only involve the pos ...

of the reduction of adenylyl-sulfate/adenosine-5'-phosphosulfate (APS) to sulfite through the use of an electron donor cofactor. The products of the reaction are AMP and sulfite, as well as an oxidized electron donor cofactor.

Nomenclature

This enzyme belongs to the family of

oxidoreductase In biochemistry, an oxidoreductase is an enzyme that catalyzes the transfer of electrons from one molecule, the reductant, also called the electron donor, to another, the oxidant, also called the electron acceptor. This group of enzymes usually ut ...

s, specifically those acting on a sulfur group of donors with other acceptors. The

systematic name A systematic name is a name given in a systematic way to one unique group, organism, object or chemical substance, out of a specific population or collection. Systematic names are usually part of a nomenclature. A semisystematic name or semitrivial ...

of this enzyme class is AMP, sulfite:acceptor oxidoreductase (adenosine-5'-phosphosulfate-forming). Other names in common use include adenosine phosphosulfate reductase, adenosine 5'-phosphosulfate reductase, APS-reductase, APS reductase, AMP, sulfite:(acceptor) oxidoreductase, and (adenosine-5'-phosphosulfate-forming). This enzyme participates in selenium metabolism and

sulfur metabolism Sulfur is metabolism, metabolized by all organisms, from bacteria and archaea to plants and animals. Sulfur is redox, reduced or redox, oxidized by organisms in a variety of forms. The chemical element, element is present in proteins, organosulfate ...

Mechanism

APS reductase catalyzes the reversible transformation of APS to sulfite and AMP, which is the rate determining step of the overall reaction. The reaction catalyzed by APS reductase is as follows:

APS + 2e^- \longleftrightarrow AMP +HSO_3^- (E^\circ = -60 mV)

Sulfate has to be activated to APS by ATP sulfurylase at the expense of one ATP, hence this reaction requires an input of energy. The reaction above occurs in a strictly anaerobic environment. The two electrons come from a reduced cofactor, in this case reduced FAD. The forward direction requires one AMP molecule; however, research suggests that the reverse reaction requires two AMP molecules (one acting on the substrate and one inhibiting the forward reaction). The reversible reaction occurs when AMP binds to the Arg317 residue, changing the confirmation of Arg317 and APS reductase as a whole, which provides the thermodynamic driving force to go in the reverse direction. APS reductases are involved in both assimilatory and dissimilatory sulfate reduction. Dissimilatory sulfate reduction takes sulfate and transforms it into sulfide, a sulfur source that can be distributed throughout the body. Assimilatory sulfate reduction takes sulfate and turns it into cysteine. Dissimilatory and assimilatory pathways both use APS reductases as a metabolic tool to produce a sulfur source and amino acids, respectively.

Structure

As of late 2014, 6

structures A structure is an arrangement and organization of interrelated elements in a material object or system, or the object or system so organized. Material structures include man-made objects such as buildings and machines and natural objects such as ...

have been solved for this class of enzymes, with PDB accession codes , , , , , and . APS Reductase Chemical Reaction Equation

The monomer of the enzyme consists of a mix of α-helices and β-sheets (both parallel and antiparallel). The protein cofactor thioredoxin can provide the required reducing equivalents for the reaction in the form of two cysteine residues, which are ultimately oxidized to a disulfide bond. The base active form of APS reductase appears to be a heterodimer, as seen in plants. In both bacteria and plants, two heterodimers tend to form together and produce a heterotetramer. The active site cleft in bacterial APS reductase has a few key elements. Residue sequences that appear to be necessary for catalysis are the P-loop (residues 60-66), the Arg-loop (residues 162-173), and the LDTG motif (residues 85-88). The P-loop, or phosphate-binding loop, is an especially important consecutive sequence of resides which aids in the recognition of the phosphate group in APS and, as a result, influences the substrate specificity for APS reductase. The C-terminal Cys256 is also catalytically essential, and seems to have a role in changing the conformation of the enzyme during catalysis. One notable chemical motif that distinguishes APS reductase from the related 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase is the presence of a conserved cysteine motif, CC-X_~80-CXXC, which occurs in addition to the universally conserved catalytic cysteine residue. This motif is correlated with the presence of a Fe-4Scluster; therefore, these iron-sulfur clusters are not present within PAPS reductase. When the iron-sulfur cluster is present, it is required for catalytic activity and coordinated to the four cysteine residues in the conserved motif on the other side of the active site cleft.

Function

Sulfur is a vital component in biological life and a key element in amino acids cysteine and methionine. APS reductase controls the rate limiting step of endogenous sulfur assimilation, which is the process of producing hydrogen sulfide from sulfite. Hydrogen sulfite is one of the major sources of sulfur in plants. APS reductase controls the flow of inorganic sulfur to cysteine, which is involved in many biological processes in plants such as growth, development, and responses to biotic and abiotic stresses. In fact, studies have shown that when cells are starved of sulfur, APS reductase gene expression fluctuates, indicating that when the plants are exposed to metabolic and regulatory stress, APS reductase is likely a crucial enzyme in producing hydrogen sulfide and restoring homeostasis. Bacteria use APS reductases to engage in assimilatory and dissimilatory sulfate reduction, which make them prime candidates to appear in wastewater treatment environments. Biofoulants can contain a number of sulfate reducing bacteria, and studies have shown that if wastewater plants are left untreated sulfate levels will decrease. These studies have further solidified APS reductase’s crucial role in the global sulfur cycle by giving organisms another unique way to obtain sulfur when it's unavailable.

Clinical significance

APS reductase does not exist within the proteome of human cells; consequently, these enzymes have become the targets of research for various environmental and medical reasons. Competitive inhibitors for the APS reductase in ''Mycobacterium tuberculosis'' have been studied as a new possible route for TB treatment, especially against drug-resistant and latent TB. Such inhibitors have also been studied in the context of obtaining oil and gas from reservoirs in order to better control the souring of such products. Some APS reductases have also been investigated for their role in selenium metabolism and reduction due to the chemical similarity between sulfur and selenium. APR2, the dominant APS reductase isozyme in the model plant ''Arabidopsis thaliana'', has been implicated in the involvement of selenate tolerance and selenite metabolism. Such research may then aid in the goal of enhancing selenium phytoremediation in plants and, as a result, dietary biofortification.

Nomenclature

Mechanism

Structure

Function

Clinical significance

References

Further reading