3-Carboxy-''cis'',''cis''-muconic acid is a metabolite of the

catechin Catechin is a flavan-3-ol, a type of secondary metabolite providing antioxidant roles in plants. It belongs to the subgroup of polyphenols called flavonoids. The name of the catechin chemical family derives from ''catechu'', which is the tannic ...

degradation by ''

Bradyrhizobium japonicum ''Bradyrhizobium japonicum'' is a species of legume- root nodulating, microsymbiotic nitrogen-fixing bacteria. The species is one of many Gram-negative, rod-shaped bacteria commonly referred to as rhizobia. Within that broad classification, wh ...

''. The enzyme 3-carboxy-''cis'',''cis''-muconate cycloisomerase uses 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate to produce 3-carboxy-''cis'',''cis''-muconate. The enzyme carboxy-''cis'',''cis''-muconate cyclase uses 3-carboxy-2,5-dihydro-5-oxofuran-2-acetate to produce 3-carboxy-''cis'',''cis''-muconate. The enzyme

protocatechuate 3,4-dioxygenase In enzymology, a protocatechuate 3,4-dioxygenase () is an enzyme that catalyzes the chemical reaction : 3,4-dihydroxybenzoate + O2 \rightleftharpoons 3-carboxy-cis,cis-muconate Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate ( ...

uses 3,4-dihydroxybenzoate and O₂ to produce 3-carboxy-''cis'',''cis''-muconate.

References

Tricarboxylic acids {{Biochem-stub