Yeast Deletion Project

	Yeast Deletion Project The yeast deletion project, formally the ''Saccharomyces'' Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast ''Saccharomyces cerevisiae''. Each strain carries a precise deletion of one of the genes in the genome. This allows researchers to determine what each gene does by comparing the mutated yeast to the behavior of normal yeast. Gene deletion, or gene knockout, is one of the main ways in which the function of genes are discovered. Many of the deletion mutations are sold by the biotech firm Invitrogen. See also * Synthetic genetic array Synthetic genetic array analysis (SGA) is a high-throughput screening, high-throughput technique for exploring synthetic lethality, synthetic lethal and synthetic sick genetic interactions (Synthetic lethality, SSL). SGA allows for the systematic co ... References * External links * http://www-deletion.stanford.edu/YDPM/YDPM_index.html * http://www-sequence.stanford. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Saccharomyces Cerevisiae ''Saccharomyces cerevisiae'' () (brewer's yeast or baker's yeast) is a species of yeast (single-celled fungus microorganisms). The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes. It is one of the most intensively studied eukaryotic model organisms in molecular biology, molecular and cell biology, much like ''Escherichia coli'' as the model bacteria, bacterium. It is the microorganism behind the most common type of fermentation (biochemistry), fermentation. ''S. cerevisiae'' cells are round to ovoid, 5–10 micrometre, μm in diameter. It reproduces by budding. Many proteins important in human biology were first discovered by studying their Homology (biology), homologs in yeast; these proteins include cell cycle proteins, signaling proteins, and protein-processing enzymes. ''S. cerevisiae'' is currently the only yeast cell known to have Berkeley body, Berkeley bo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]
picture info	Gene Knockout A gene knockout (abbreviation: KO) is a genetic technique in which one of an organism's genes is made inoperative ("knocked out" of the organism). However, KO can also refer to the gene that is knocked out or the organism that carries the gene knockout. Knockout organisms or simply knockouts are used to study gene function, usually by investigating the effect of gene loss. Researchers draw inferences from the difference between the knockout organism and normal individuals. The KO technique is essentially the opposite of a gene knock-in. Knocking out two genes simultaneously in an organism is known as a double knockout (DKO). Similarly the terms triple knockout (TKO) and quadruple knockouts (QKO) are used to describe three or four knocked out genes, respectively. However, one needs to distinguish between heterozygous and homozygous KOs. In the former, only one of two gene copies (alleles) is knocked out, in the latter both are knocked out. Methods Knockouts are accomplished throu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu]