Transmission Electron Microscopy
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Transmission Electron Microscopy
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device. Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons. This enables the instrument to capture fine detail—even as small as a single column of atoms, which is thousands of times smaller than a resolvable object seen in a light microscope. Transmission electron microscopy is a major analytical method i ...
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Polio EM PHIL 1875 Lores
Poliomyelitis, commonly shortened to polio, is an infectious disease caused by the poliovirus. Approximately 70% of cases are asymptomatic; mild symptoms which can occur include sore throat and fever; in a proportion of cases more severe symptoms develop such as headache, neck stiffness, and paresthesia. These symptoms usually pass within one or two weeks. A less common symptom is permanent paralysis, and possible death in extreme cases.. Years after recovery, post-polio syndrome may occur, with a slow development of muscle weakness similar to that which the person had during the initial infection. Polio occurs naturally only in humans. It is highly infectious, and is spread from person to person either through fecal-oral transmission (e.g. poor hygiene, or by ingestion of food or water contaminated by human feces), or via the oral-oral route. Those who are infected may spread the disease for up to six weeks even if no symptoms are present. The disease may be diagnosed by f ...
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Semiconductor
A semiconductor is a material which has an electrical resistivity and conductivity, electrical conductivity value falling between that of a electrical conductor, conductor, such as copper, and an insulator (electricity), insulator, such as glass. Its electrical resistivity and conductivity, resistivity falls as its temperature rises; metals behave in the opposite way. Its conducting properties may be altered in useful ways by introducing impurities ("doping (semiconductor), doping") into the crystal structure. When two differently doped regions exist in the same crystal, a semiconductor junction is created. The behavior of charge carriers, which include electrons, ions, and electron holes, at these junctions is the basis of diodes, transistors, and most modern electronics. Some examples of semiconductors are silicon, germanium, gallium arsenide, and elements near the so-called "metalloid staircase" on the periodic table. After silicon, gallium arsenide is the second-most common s ...
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Julius Plücker
Julius Plücker (16 June 1801 – 22 May 1868) was a German mathematician and physicist. He made fundamental contributions to the field of analytical geometry and was a pioneer in the investigations of cathode rays that led eventually to the discovery of the electron. He also vastly extended the study of Lamé curves. Biography Early years Plücker was born at Elberfeld (now part of Wuppertal). After being educated at Düsseldorf and at the universities of Bonn, Heidelberg and Berlin he went to Paris in 1823, where he came under the influence of the great school of French geometers, whose founder, Gaspard Monge, had only recently died. In 1825 he returned to Bonn, and in 1828 was made professor of mathematics. In the same year he published the first volume of his ''Analytisch-geometrische Entwicklungen'', which introduced the method of "abridged notation". In 1831 he published the second volume, in which he clearly established on a firm and independent basis projective ...
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Moritz Von Rohr
Moritz von Rohr (4 April 1868 – 20 June 1940) was an optical scientist at Carl Zeiss in Jena, Germany. A street in Jena is named after him: Moritz-von-Rohr-Straße, near Carl-Zeiss-Promenade and Otto-Schott-Straße. Life Moritz von Rohr was born in Lonzyn near Hohensalza, then part of the Prussian Grand Duchy of Posen, but now in Poland and known as Łążyn, near Inowrocław. He obtained a doctorate of philosophy at the University of Berlin in 1892. Inventions Von Rohr is usually credited with the design of the first aspheric lenses for eyeglasses. He invented the eyeglass lens designs that became the Zeiss Punktal lenses. He also developed a method of computing depth of field from a camera's entrance pupil location and diameter, without reference to focal length and f-number In optics, the f-number of an optical system such as a camera lens is the ratio of the system's focal length to the diameter of the entrance pupil ("clear aperture").Smith, Warren ' ...
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August Köhler
August Karl Johann Valentin Köhler (4 March 1866 – 12 March 1948) was a German professor and early staff member of Carl Zeiss AG in Jena, Germany. He is best known for his development of the microscopy technique of Köhler illumination, an important principle in optimizing microscopic resolution power by evenly illuminating the field of view. This invention revolutionized light microscope design and is widely used in traditional as well as modern digital imaging techniques today. Early life and education Köhler was born in 1866 in Darmstadt, Germany, where he attended the Ludwig-Georgs-Gymnasium until 1884. He studied at the Technical University in Darmstadt and at the universities of Heidelberg and Giessen covering a wide range of fields from zoology and botany to mineralogy, physics, and chemistry. Teaching and academic career In 1888, August Köhler graduated with a teaching degree and subsequently taught at gymnasiums in Darmstadt and Bingen before going back to univers ...
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Ultraviolet
Ultraviolet (UV) is a form of electromagnetic radiation with wavelength from 10 nanometer, nm (with a corresponding frequency around 30 Hertz, PHz) to 400 nm (750 Hertz, THz), shorter than that of visible light, but longer than X-rays. UV radiation is present in sunlight, and constitutes about 10% of the total electromagnetic radiation output from the Sun. It is also produced by electric arcs and specialized lights, such as mercury-vapor lamps, tanning lamps, and black lights. Although long-wavelength ultraviolet is not considered an ionizing radiation because its photons lack the energy to ionization, ionize atoms, it can cause chemical reactions and causes many substances to glow or fluorescence, fluoresce. Consequently, the chemical and biological effects of UV are greater than simple heating effects, and many practical applications of UV radiation derive from its interactions with organic molecules. Short-wave ultraviolet light damages DNA and sterilizes surf ...
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Wavelength
In physics, the wavelength is the spatial period of a periodic wave—the distance over which the wave's shape repeats. It is the distance between consecutive corresponding points of the same phase on the wave, such as two adjacent crests, troughs, or zero crossings, and is a characteristic of both traveling waves and standing waves, as well as other spatial wave patterns. The inverse of the wavelength is called the spatial frequency. Wavelength is commonly designated by the Greek letter ''lambda'' (λ). The term ''wavelength'' is also sometimes applied to modulated waves, and to the sinusoidal envelopes of modulated waves or waves formed by interference of several sinusoids. Assuming a sinusoidal wave moving at a fixed wave speed, wavelength is inversely proportional to frequency of the wave: waves with higher frequencies have shorter wavelengths, and lower frequencies have longer wavelengths. Wavelength depends on the medium (for example, vacuum, air, or water) that a wav ...
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Diffraction Limit
The resolution of an optical imaging system a microscope, telescope, or camera can be limited by factors such as imperfections in the lenses or misalignment. However, there is a principal limit to the resolution of any optical system, due to the physics of diffraction. An optical system with resolution performance at the instrument's theoretical limit is said to be diffraction-limited. The diffraction-limited angular resolution of a telescopic instrument is inversely proportional to the wavelength of the light being observed, and proportional to the diameter of its objective's entrance aperture. For telescopes with circular apertures, the size of the smallest feature in an image that is diffraction limited is the size of the Airy disk. As one decreases the size of the aperture of a telescopic lens, diffraction proportionately increases. At small apertures, such as f/22, most modern lenses are limited only by diffraction and not by aberrations or other imperfections in the co ...
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Ernst Abbe
Ernst Karl Abbe HonFRMS (23 January 1840 – 14 January 1905) was a German physicist, optical scientist, entrepreneur, and social reformer. Together with Otto Schott and Carl Zeiss, he developed numerous optical instruments. He was also a co-owner of Carl Zeiss AG, a German manufacturer of scientific microscopes, astronomical telescopes, planetariums, and other advanced optical systems. Personal life Abbe was born 23 January 1840 in Eisenach, Saxe-Weimar-Eisenach, to Georg Adam Abbe and Elisabeth Christina Barchfeldt. He came from a humble home – his father was a foreman in a spinnery. Supported by his father's employer, Abbe was able to attend secondary school and to obtain the general qualification for university entrance with fairly good grades, at the Eisenach Gymnasium, which he graduated from in 1857. By the time he left school, his scientific talent and his strong will had already become obvious. Thus, in spite of the family's strained financial situation, his father ...
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Ernst Ruska Electron Microscope - Deutsches Museum - Munich-edit
Ernst is both a surname and a given name, the German, Dutch, and Scandinavian form of Ernest. Notable people with the name include: Surname * Adolf Ernst (1832–1899) German botanist known by the author abbreviation "Ernst" * Anton Ernst (1975-) South African Film Producer * Alice Henson Ernst (1880-1980), American writer and historian * Britta Ernst (born 1961), German politician * Cornelia Ernst, German politician * Edzard Ernst, German-British Professor of Complementary Medicine * Emil Ernst, astronomer * Ernie Ernst (1924/25–2013), former District Judge in Walker County, Texas * Eugen Ernst (1864–1954), German politician * Fabian Ernst, German soccer player * Gustav Ernst, Austrian writer * Heinrich Wilhelm Ernst, Moravian violinist and composer * Jim Ernst, Canadian politician * Jimmy Ernst, American painter, son of Max Ernst * Joni Ernst, U.S. Senator from Iowa * K.S. Ernst, American visual poet * Karl Friedrich Paul Ernst, German writer (1866–1933) * Ken Ernst, U.S ...
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Ernst Ruska
Ernst August Friedrich Ruska (; 25 December 1906 – 27 May 1988) was a German physicist who won the Nobel Prize in Physics in 1986 for his work in electron optics, including the design of the first electron microscope. Life and career Ernst Ruska was born in Heidelberg, Germany. He was educated at the Technical University of Munich from 1925 to 1927 and then entered the Technical University of Berlin, where he posited that microscopes using electrons, with wavelengths 1000 times shorter than those of light, could provide a more detailed picture of an object than a microscope utilizing light, in which magnification is limited by the size of the wavelengths. In 1931, he demonstrated that a magnetic coil could act as an electron lens, and used several coils in a series to build the first electron microscope in 1933. After completing his PhD in 1933, Ruska continued to work in the field of electron optics, first at Fernseh AG in Berlin-Zehlendorf, and then from 1937 at Siemens ...
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