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Transgenics
A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. The exact definition of a genetically modified organism and what constitutes genetic engineering varies, with the most common being an organism altered in a way that "does not occur naturally by mating and/or natural recombination". A wide variety of organisms have been genetically modified (GM), from animals to plants and microorganisms. Genes have been transferred within the same species, across species (creating transgenic organisms), and even across kingdoms. New genes can be introduced, or endogenous genes can be enhanced, altered, or knocked out. Creating a genetically modified organism is a multi-step process. Genetic engineers must isolate the gene they wish to insert into the host organism and combine it with other genetic elements, including a promoter and terminator region and often a selectable marker. A number of techniques are a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genetically Modified Animal
Genetically modified animals are animals that have been genetically modified for a variety of purposes including producing drugs, enhancing yields, increasing resistance to disease, etc. The vast majority of genetically modified animals are at the research stage while the number close to entering the market remains small. Production The process of genetically engineering mammals is a slow, tedious, and expensive process.Murray, Joo (20)Genetically modified animals Canada: Brainwaving As with other genetically modified organisms (GMOs), first genetic engineers must isolate the gene they wish to insert into the host organism. This can be taken from a cell containing the gene or artificially synthesised. If the chosen gene or the donor organism's genome has been well studied it may already be accessible from a genetic library. The gene is then combined with other genetic elements, including a promoter and terminator region and usually a selectable marker. A number of technique ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rudolf Jaenisch
Rudolf Jaenisch (born April 22, 1942) is a Professor of Biology at MIT and a founding member of the Whitehead Institute for Biomedical Research. He is a pioneer of transgenic science, in which an animal’s genetic makeup is altered. Jaenisch has focused on creating genetically modified mice to study cancer and neurological diseases. Research Jaenisch’s first breakthrough occurred in 1974 when he and Beatrice Mintz showed that foreign DNA could be integrated into the DNA of early mouse embryos. They injected retrovirus DNA into early mouse embryos and showed that leukemia DNA sequences had integrated into the mouse genome and also to its offspring. These mice were the first transgenic mammals in history. His current research focuses on the epigenetic regulation of gene expression, which has led to major advances in creating embryonic stem cells and “ induced pluripotent stem" (IPS) cells, as well as their therapeutic applications. In 2007, Jaenisch’s laboratory was o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genetically Modified Food
Genetically modified foods (GM foods), also known as genetically engineered foods (GE foods), or bioengineered foods are foods produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Genetic engineering techniques allow for the introduction of new traits as well as greater control over traits when compared to previous methods, such as selective breeding and mutation breeding. The discovery of DNA and the improvement of genetic technology in the 20th century played a crucial role in the development of transgenic technology. In 1988, genetically modified microbial enzymes were first approved for use in food manufacture. Recombinant rennet was used in few countries in the 1990s. Commercial sale of genetically modified foods began in 1994, when Calgene first marketed its unsuccessful Flavr Savr delayed-ripening tomato.Weasel, Lisa H. 2009. ''Food Fray''. Amacom Publishing Most food modifications have primarily focused on cash ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gene Delivery
Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Gene delivery must reach the genome of the host cell to induce gene expression. Successful gene delivery requires the foreign gene delivery to remain stable within the host cell and can either integrate into the genome or replicate independently of it. This requires foreign DNA to be synthesized as part of a vector, which is designed to enter the desired host cell and deliver the transgene to that cell's genome. Vectors utilized as the method for gene delivery can be divided into two categories, recombinant viruses and synthetic vectors (viral and non-viral). In complex multicellular eukaryotes (more specifically Weissmanists), if the transgene is incorporated into the host's germline cells, the resulting host cell can pass the transgene to its progeny. If the transgene is incorporated into somatic cells, the transgene will stay with the somatic cell line, and thus its h ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gene Therapy
Gene therapy is a medical field which focuses on the genetic modification of cells to produce a therapeutic effect or the treatment of disease by repairing or reconstructing defective genetic material. The first attempt at modifying human DNA was performed in 1980, by Martin Cline, but the first successful nuclear gene transfer in humans, approved by the National Institutes of Health, was performed in May 1989. The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September 1990. It is thought to be able to cure many genetic disorders or treat them over time. Between 1989 and December 2018, over 2,900 clinical trials were conducted, with more than half of them in phase I.Gene Therapy Cl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Viral Vector
Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (''in vivo'') or in cell culture (''in vitro''). Viruses have evolved specialized molecular mechanisms to efficiently transport their genomes inside the cells they infect. Delivery of genes or other genetic material by a vector is termed transduction and the infected cells are described as transduced. Molecular biologists first harnessed this machinery in the 1970s. Paul Berg used a modified SV40 virus containing DNA from the bacteriophage λ to infect monkey kidney cells maintained in culture. In addition to their use in molecular biology research, viral vectors are used for gene therapy and the development of vaccines. Key properties of a viral vector VIRAL VECTORS are tailored to their specific applications but generally share a few key properties. *''Safety'': Although viral vectors are occasionally created from path ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
AquAdvantage Salmon
AquAdvantage salmon is a genetically engineered (GE) fish, a GE Atlantic salmon developed by AquaBounty Technologies in 1989. The typical growth hormone-regulating gene in the Atlantic salmon was replaced with the growth hormone-regulating gene from Pacific Chinook salmon, with a promoter sequence from ocean pout. This gene enables the GM salmon to grow year-round instead of only during spring and summer. These GE salmon are a commercially competitive alternative to wild-caught salmon and to fish farming of unmodified salmon. The purpose of the modifications is to increase the speed at which the fish grows without affecting its ultimate size or other qualities. Fish-farmed Atlantic salmon growth rates have already been improved over wild fish as a result of traditional selective breeding practices. However, GM fish are able to grow even faster and grow to market size in just 16 to 18 months rather than three years. Significance AquAdvantage salmon are the first genetically e ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
GloFish
The GloFish is a patented and trademarked brand of genetically engineered fluorescent fish. They have been created from several different species of fish: zebrafish (''Danio rerio'') were the first GloFish available in pet stores, and recently tetra (''Gymnocorymbus ternetzi''), tiger barbs (''Puntius tetrazona''), Rainbow Shark ('' Epalzeorhynchos frenatum)'', Siamese fighting fish (''Betta splendens''), and most recently Bronze corydoras (''Corydoras aeneus'') have been added to the lineup. They are sold in many colors, trademarked as "Starfire Red", "Moonrise Pink", "Sunburst Orange", "Electric Green", "Cosmic Blue", and "Galactic Purple", although not all species are available in all colors. Although not originally developed for the ornamental fish trade, it is one of the first genetically modified animals to become publicly available. The rights to GloFish are owned by Spectrum Brands, Inc., which purchased GloFish from Yorktown Technologies, the original developer of GloFis ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flavr Savr
Flavr Savr (also known as CGN-89564-2; pronounced "flavor saver"), a genetically modified tomato, was the first commercially grown genetically engineered food to be granted a license for human consumption. It was developed by the Californian company Calgene in the 1980s. The tomato has an improved shelf-life, increased fungal resistance and a slightly increased viscosity compared to its non-modified counterpart. It was meant to be harvested ripe for increased flavor for long-distance shipping. The Flavr Savr contains two genes added by Calgene; a reversed antisense polygalacturonase gene which inhibits the production of the aforementioned rotting enzyme and a gene responsible for the creation of APH(3')II, which confers resistance to certain aminoglycoside antibiotics including kanamycin and neomycin. On May 18, 1994, the FDA completed its evaluation of the Flavr Savr tomato and the use of APH(3')II, concluding that the tomato "is as safe as tomatoes bred by conventional means" and " ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kanamycin
Kanamycin A, often referred to simply as kanamycin, is an antibiotic used to treat severe bacterial infections and tuberculosis. It is not a first line treatment. It is used by mouth, injection into a vein, or injection into a muscle. Kanamycin is recommended for short-term use only, usually from 7 to 10 days. As with most antibiotics, it is ineffective in viral infections. Common side effects include hearing and balance problems. Kidney problems may also occur. Kanamycin is not recommended during pregnancy as it may harm the baby. It is likely safe during breastfeeding. Kanamycin is in the aminoglycoside family of medications. It works by blocking the production of proteins that are required for bacterial survival. Kanamycin was first isolated in 1957 by Hamao Umezawa from the bacterium '' Streptomyces kanamyceticus''. It was removed from the World Health Organization's List of Essential Medicines in 2019. It is no longer marketed in the United States. Medical uses Spe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stanley Norman Cohen
Stanley Norman Cohen (born February 17, 1935) is an American geneticist and the Kwoh-Ting Li Professor in the Stanford University School of Medicine. Stanley Cohen and Herbert Boyer were the first scientists to transplant genes from one living organism to another, a fundamental discovery for genetical engineering. Thousands of products have been developed on the basis of their work, including human growth hormone and hepatitis B vaccine. According to immunologist Hugh McDevitt, "Cohen's DNA cloning technology has helped biologists in virtually every field". Without it, "the face of biomedicine and biotechnology would look totally different." Boyer cofounded Genentech in 1976 based on their work together, but Cohen was a consultant for Cetus Corporation and declined to join. Early life Cohen was born in Perth Amboy, New Jersey. He graduated from Rutgers University with a B.S. in 1956, and received his M.D. from the University of Pennsylvania School of Medicine in 1960. Cohen th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
