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Standard Addition
The Standard addition method, also called known addition, often used in analytical chemistry, quantifies the analyte present in an unknown. This method is useful for analyzing complex samples where a matrix effect interferes with the analyte signal. In comparison to the calibration curve method, the standard addition method has the advantage of the matrices of the unknown and standards being nearly identical. This minimizes the potential bias arising from the matrix effect when determining the concentration. Variations Standard addition involves adding known amounts of analyte to an unknown sample, a process known as ''spiking''. By increasing the number of spikes, the analyst can extrapolate for the analyte concentration in the unknown that has not been spiked. There are multiple approaches to the standard addition. The following section summarize each approach. Single standard addition used in polarography In classic polarography, the standard addition method involves creati ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Analytical Chemistry
Analytical skill, Analytical chemistry studies and uses instruments and methods to Separation process, separate, identify, and Quantification (science), quantify matter. In practice, separation, identification or quantification may constitute the entire analysis or be combined with another method. Separation isolates analytes. Qualitative inorganic analysis, Qualitative analysis identifies analytes, while Quantitative analysis (chemistry), quantitative analysis determines the numerical amount or concentration. Analytical chemistry consists of classical, wet chemistry, wet chemical methods and modern analytical techniques. Classical qualitative methods use separations such as Precipitation (chemistry), precipitation, Extraction (chemistry), extraction, and distillation. Identification may be based on differences in color, odor, melting point, boiling point, solubility, radioactivity or reactivity. Classical quantitative analysis uses mass or volume changes to quantify amount. Ins ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Standard Addition Example
Standard may refer to: Symbols * Colours, standards and guidons, kinds of military signs * Standard (emblem), a type of a large symbol or emblem used for identification Norms, conventions or requirements * Standard (metrology), an object that bears a defined relationship to a unit of measure used for calibration of measuring devices * Standard (timber unit), an obsolete measure of timber used in trade * Breed standard (also called bench standard), in animal fancy and animal husbandry * BioCompute Standard, a standard for next generation sequencing * ''De facto'' standard, product or system with market dominance * Gold standard, a monetary system based on gold; also used metaphorically for the best of several options, against which the others are measured * Internet Standard, a specification ratified as an open standard by the Internet Engineering Task Force * Learning standards, standards applied to education content * Standard displacement, a naval term describing the weig ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Internal Standard
In a chemical analysis, the internal standard method involves adding the same amount of a chemical substance to each sample and calibration solution. The internal standard responds proportionally to changes in the analyte and provides a similar, but not identical, measurement signal. It must also be absent from the sample matrix to ensure there is no other source of the internal standard present. Taking the ratio of analyte signal to internal standard signal and plotting it against the analyte concentrations in the calibration solutions will result in a calibration curve. The calibration curve can then be used to calculate the analyte concentration in an unknown sample. Selecting an appropriate internal standard accounts for random and systematic sources of uncertainty that arise during sample preparation or instrument fluctuation. This is because the ratio of analyte relative to the amount of internal standard is independent of these variations. If the measured value of the analyt ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Isotope Dilution
Isotope dilution analysis is a method of determining the quantity of chemical substances. In its most simple conception, the method of isotope dilution comprises the addition of known amounts of isotopically enriched substance to the analyzed sample. Mixing of the isotopic standard with the sample effectively "dilutes" the isotopic enrichment of the standard and this forms the basis for the isotope dilution method. Isotope dilution is classified as a method of internal standardisation, because the standard (isotopically enriched form of analyte) is added directly to the sample. In addition, unlike traditional analytical methods which rely on signal intensity, isotope dilution employs signal ratios. Owing to both of these advantages, the method of isotope dilution is regarded among chemistry measurement methods of the highest metrological standing. Isotopes are variants of a particular chemical element which differ in neutron number. All isotopes of a given element have the same nu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Standard Curve
In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. A calibration curve is one approach to the problem of instrument calibration; other standard approaches may mix the standard into the unknown, giving an internal standard. The calibration curve is a plot of how the instrumental response, the so-called analytical signal, changes with the concentration of the analyte (the substance to be measured). General use In more general use, a calibration curve is a curve or table for a measuring instrument which measures some parameter indirectly, giving values for the desired quantity as a function of values of sensor output. For example, a calibration curve can be made for a particular pressure transducer to determine applied pressure from transducer output (a voltage). Such a curve is typicall ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Least Squares
The method of least squares is a mathematical optimization technique that aims to determine the best fit function by minimizing the sum of the squares of the differences between the observed values and the predicted values of the model. The method is widely used in areas such as regression analysis, curve fitting and data modeling. The least squares method can be categorized into linear and nonlinear forms, depending on the relationship between the model parameters and the observed data. The method was first proposed by Adrien-Marie Legendre in 1805 and further developed by Carl Friedrich Gauss. History Founding The method of least squares grew out of the fields of astronomy and geodesy, as scientists and mathematicians sought to provide solutions to the challenges of navigating the Earth's oceans during the Age of Discovery. The accurate description of the behavior of celestial bodies was the key to enabling ships to sail in open seas, where sailors could no longer rely on la ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Linear Regression
In statistics, linear regression is a statistical model, model that estimates the relationship between a Scalar (mathematics), scalar response (dependent variable) and one or more explanatory variables (regressor or independent variable). A model with exactly one explanatory variable is a ''simple linear regression''; a model with two or more explanatory variables is a multiple linear regression. This term is distinct from multivariate linear regression, which predicts multiple correlated dependent variables rather than a single dependent variable. In linear regression, the relationships are modeled using linear predictor functions whose unknown model parameters are estimation theory, estimated from the data. Most commonly, the conditional mean of the response given the values of the explanatory variables (or predictors) is assumed to be an affine function of those values; less commonly, the conditional median or some other quantile is used. Like all forms of regression analysis, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inductively Coupled Plasma Mass Spectrometry
Inductively coupled plasma mass spectrometry (ICP-MS) is a type of mass spectrometry that uses an inductively coupled plasma to ionize the sample. It atomizes the sample and creates atomic and small polyatomic ions, which are then detected. It is known and used for its ability to detect metals and several non-metals in liquid samples at very low concentrations. It can detect different isotopes of the same element, which makes it a versatile tool in isotopic labeling. Compared to atomic absorption spectroscopy, ICP-MS has greater speed, precision, and sensitivity. However, compared with other types of mass spectrometry, such as thermal ionization mass spectrometry (TIMS) and glow discharge mass spectrometry (GD-MS), ICP-MS introduces many interfering species: argon from the plasma, component gases of air that leak through the cone orifices, and contamination from glassware and the cones. Components Inductively coupled plasma An inductively coupled plasma is a plasma that is ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Aqueous Solution
An aqueous solution is a solution in which the solvent is water. It is mostly shown in chemical equations by appending (aq) to the relevant chemical formula. For example, a solution of table salt, also known as sodium chloride (NaCl), in water would be represented as . The word ''aqueous'' (which comes from ''aqua'') means pertaining to, related to, similar to, or dissolved in, water. As water is an excellent solvent and is also naturally abundant, it is a ubiquitous solvent in chemistry. Since water is frequently used as the solvent in experiments, the word solution refers to an aqueous solution, unless the solvent is specified. A ''non-aqueous solution'' is a solution in which the solvent is a liquid, but is not water. Characteristics Substances that are ''hydrophobic'' ('water-fearing') do not dissolve well in water, whereas those that are '' hydrophilic'' ('water-friendly') do. An example of a hydrophilic substance is sodium chloride. In an aqueous solution the hydrogen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Matrix Effect
In chemical analysis, matrix refers to the components of a sample other than the analyte of interest. The matrix can have a considerable effect on the way the analysis is conducted and the quality of the results are obtained; such effects are called matrix effects.F. W. Fifield, P. J. Haines. ''Environmental Analytical Chemistry''. Blackwell Publishing, 2000, p. 4-5. . For example, the ionic strength of the solution can have an effect on the activity coefficients of the analytes.Harris, D. C. ''Quantitative Chemical Analysis'', 4th ed. Freeman, 1995, pp.194, 404. . The most common approach for accounting for matrix effects is to build a calibration curve using standard samples with known analyte concentration and which try to approximate the matrix of the sample as much as possible. This is especially important for solid samples where there is a strong matrix influence.Marco Aurelio Zezzi Arruda. ''Trends in Sample Preparation''. Nova Publishers, 2006, p. 15-18. . In cases with comp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spectrometer
A spectrometer () is a scientific instrument used to separate and measure Spectrum, spectral components of a physical phenomenon. Spectrometer is a broad term often used to describe instruments that measure a continuous variable of a phenomenon where the spectral components are somehow mixed. In visible light a spectrometer can separate white light and measure individual narrow bands of color, called a spectrum. A mass spectrometer measures the spectrum of the masses of the atoms or molecules present in a gas. The first spectrometers were used to split light into an array of separate colors. Spectrometers were History_of_spectroscopy, developed in early studies of physics, astronomy, and chemistry. The capability of spectroscopy to determine Analytical_chemistry#Spectroscopy, chemical composition drove its advancement and continues to be one of its primary uses. Spectrometers are used in Astronomical spectroscopy, astronomy to analyze the chemical composition of Astronomical_spe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photographic Film
Photographic film is a strip or sheet of transparent film base coated on one side with a gelatin photographic emulsion, emulsion containing microscopically small light-sensitive silver halide crystals. The sizes and other characteristics of the crystals determine the sensitivity, contrast, and image resolution, resolution of the film. Film is typically segmented in ''frames'', that give rise to separate photographs. The emulsion will gradually darken if left exposed to light, but the process is too slow and incomplete to be of any practical use. Instead, a very short exposure (photography), exposure to the image formed by a camera lens is used to produce only a very slight chemical change, proportional to the amount of light absorbed by each crystal. This creates an invisible latent image in the emulsion, which can be chemically photographic processing, developed into a visible photograph. In addition to visible light, all films are sensitive to ultraviolet light, X-rays, gamma ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
