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Streak Plate Method
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested. The modern streak plate method has progressed from the efforts of Robert Koch and other microbiologists to obtain microbiological cultures of bacteria in order to study them. The dilution or isolation by streaking method was first developed by Loeffler and Gaffky in Koch's laboratory, which involves the dilution of bacteria by systematically streaking them over the exterior of the agar in a Petri dish to obtain isolated colonies which will then grow into quantity of cells, or isolated colonies. If the agar surface grows microorganisms which are all genetically same, the culture is then considered as a microbiological culture. Technique Streaking is rapid and ideally a simple process ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Legionella Plate 01
''Legionella'' is a genus of pathogenic gram-negative bacteria that includes the species '' L. pneumophila'', causing legionellosis (all illnesses caused by ''Legionella'') including a pneumonia-type illness called Legionnaires' disease and a mild flu-like illness called Pontiac fever. ''Legionella'' may be visualized with a silver stain or cultured in cysteine-containing media such as buffered charcoal yeast extract agar. It is common in many environments, including soil and aquatic systems, with at least 50 species and 70 serogroups identified. These bacteria, however, are not transmissible from person to person; furthermore, most people exposed to the bacteria do not become ill. Most outbreaks are traced to poorly maintained cooling towers. The side chains of the cell wall carry the bases responsible for the somatic antigen specificity of these organisms. The chemical composition of these side chains both with respect to components and arrangement of the different sugars det ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inoculation Loop
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate.Ronald Westphal (1988): Microbiological Techniques in School', page 34. Document No. 28 in the series ''Science and Technology Education''. This process is called inoculation. The tool consists of a thin handle with a loop about 5 mm wide or smaller at the end. It was originally made of twisted metal wire (such as platinum, tungsten or nichrome), but disposable molded plastic versions are now common. See also * Cell spreader * Micropipette A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with diff ... References Laborat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacterial Lawn
Bacterial lawn is a term used by microbiologists to describe the appearance of bacterial colonies when all the individual colonies on a Petri dish agar plate merge to form a field or mat of bacteria. Bacterial lawns find use in screens for antibiotic resistance and bacteriophage titering. Bacterial lawns (often of ''Serratia marcescens'') are also used extensively when as an assay method when using bacteriophage as tracers in studies of groundwater flow. Although occasionally used as a synonym for biofilm, the term primarily applies to the simple, clonal, unstructured mats of organisms that typically only form on laboratory growth media. Biofilms—the aggregated form of microorganisms most commonly found in nature— are generally more complex and diverse and marked by larger quantities of extracellular structural matrix relative to the cellular biomass. Techniques Bacterial lawns can be produced manually by evenly spreading a high amount of bacteria onto an agar plat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacterium
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationshi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Normal Flora
The human microbiome is the aggregate of all microbiota that reside on or within human tissues and biofluids along with the corresponding anatomical sites in which they reside, including the skin, mammary glands, seminal fluid, uterus, ovarian follicles, lung, saliva, oral mucosa, conjunctiva, biliary tract, and gastrointestinal tract. Types of human microbiota include bacteria, archaea, fungi, protists and viruses. Though micro-animals can also live on the human body, they are typically excluded from this definition. In the context of genomics, the term ''human microbiome'' is sometimes used to refer to the collective genomes of resident microorganisms; however, the term '' human metagenome'' has the same meaning. Humans are colonized by many microorganisms, with approximately the same order of magnitude of non-human cells as human cells. Some microorganisms that colonize humans are commensal, meaning they co-exist without harming humans; others have a mutualistic relationship ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Incubator (microbiology)
An incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the CO2 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells. An incubator is made up of a chamber with a regulated temperature. Some incubators also regulate humidity, gas composition, or ventilation within that chamber. The simplest incubators are insulated boxes with an adjustable heater, typically going up to 60 to 65 °C (140 to 150 °F), though some can go slightly higher (generally to no more than 100 °C). The most commonly used temperature both for bacteria such as the frequently used E. coli as well as for mammalian cells is approximately 37 °C (99 °F), as these organisms grow well under such conditions. For other organisms used in bio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Death
Cell death is the event of a biological cell ceasing to carry out its functions. This may be the result of the natural process of old cells dying and being replaced by new ones, as in programmed cell death, or may result from factors such as diseases, localized injury, or the death of the organism of which the cells are part. Apoptosis or Type I cell-death, and autophagy or Type II cell-death are both forms of programmed cell death, while necrosis is a non-physiological process that occurs as a result of infection or injury. Programmed cell death Programmed cell death (PCD) is cell death mediated by an intracellular program. PCD is carried out in a regulated process, which usually confers advantage during an organism's life-cycle. For example, the differentiation of fingers and toes in a developing human embryo occurs because cells between the fingers apoptose; the result is that the digits separate. PCD serves fundamental functions during both plant and metazoa (multicellula ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nutrient Agar
Nutrient agar is a general purpose liquid medium supporting growth of a wide range of non-fastidious organisms. It typically contains ( mass/volume): * 0.5% peptone - this provides organic nitrogen * 0.3% beef extract/yeast extract - the water-soluble content of these contribute vitamins, carbohydrates, nitrogen, and salts * 1.5% agar - this gives the mixture solidity * 0.5% sodium chloride - this gives the mixture proportions similar to those found in the cytoplasm of most organisms * distilled water - water serves as a transport medium for the agar's various substances * pH adjusted to neutral (6.8) at . Nutrient broth has the same composition,but lacks agar. These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at for 15 minutes. Then they are cooled to around and poured into Petri dishes which are covered immediately. Once the dishes hold solidified agar, they are stored upside d ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Inoculum
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In biology, inoculum refers to the source material used for inoculation. ''Inoculum'' may refer to: * In medicine, material that is the source of the inoculation in a vaccine * In microbiology, propagules: cells, tissue, or viruses that are used to inoculate a new culture * Microbial inoculant, the beneficial introduction of microbes to improve plant health * A method of propagation of fungal plant disease transmission * Fermentation starter, in food production See also: * ''Fear Inoculum'', a 2019 album by American rock band Tool A tool is an object that can extend an individual's ability to modify features of the surrounding environment or help them accomplish a particular task. Although many animals use simple tools, only human beings, whose use of stone tools dates b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
FINAL Mmg 301 Honors Option
Final, Finals or The Final may refer to: *Final (competition), the last or championship round of a sporting competition, match, game, or other contest which decides a winner for an event ** Another term for playoffs, describing a sequence of contests taking place after a regular season or round-robin tournament, culminating in a final by the first definition. *final (Java), a keyword in the Java programming language *Final case, a grammatical case *Final examination or finals, a test given at the end of a course of study or training *Part of a syllable *Final, a tone of the Gregorian mode Art and entertainment * ''Final'' (film), a science fiction film * ''The Final'' (film), a thriller film * ''Finals'' (film), a 2019 Malayalam sports drama film *Final (band), an English electronic musical group * ''Final'' (Vol. 1), album by Enrique Iglesias * ''The Final'' (album), by Wham! *"The Final", a song by Dir en grey on the album ''Withering to Death'' * ''Finals'' (comics), a four-is ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Growth Medium
A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation or small plants like the moss ''Physcomitrella patens''. Different types of media are used for growing different types of cells. The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and those used for microbiological culture, which are used for growing microorganisms such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells. Types The most common growth media ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
