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Rhamnogalacturonan Endolyase
The enzyme Rhamnogalacturonan endolyase (, ''rhamnogalacturonase B'', ''α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase'', ''Rgase B'', ''rhamnogalacturonan α-Lrhamnopyranosyl-(1,4)-α-D-galactopyranosyluronide lyase'', ''RG-lyase'', ''YesW'', ''RGL4'', ''Rgl11A'', ''Rgl11Y'', ''RhiE'') is an enzyme with systematic name ''α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronate endolyase''. catalyses the following process: : Endotype eliminative cleavage of L-α-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated Dgalactopyranosyluronic acid at the non-reducing end. The enzyme is part of the degradation system for rhamnogalacturonan I in '' Bacillus subtilis'' strain 168 and '' Aspergillus aculeatus''. References External links * {{Portal bar, Biology, border=no EC 4.2.2 ...
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Enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps. The study of enzymes is called ''enzymology'' and the field of pseudoenzyme analysis recognizes that during evolution, some enzymes have lost the ability to carry out biological catalysis, which is often reflected in their amino acid sequences and unusual 'pseudocatalytic' properties. Enzymes are known to catalyze more than 5,000 biochemical reaction types. Other biocatalysts are catalytic RNA molecules, called ribozymes. Enzymes' specificity comes from their unique three-dimensional structures. Like all catalysts, enzymes increase the reaction ra ...
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List Of Enzymes
This article lists enzymes by their classification in the International Union of Biochemistry and Molecular Biology's Enzyme Commission (EC) numbering system. * List of EC numbers (EC 5) * List of EC numbers (EC 6) :Oxidoreductases (EC 1) (Oxidoreductase) *Dehydrogenase * Luciferase *DMSO reductase :EC 1.1 (act on the CH-OH group of donors) * :EC 1.1.1 (with NAD+ or NADP+ as acceptor) ** Alcohol dehydrogenase (NAD) ** Alcohol dehydrogenase (NADP) **Homoserine dehydrogenase ** Aminopropanol oxidoreductase **Diacetyl reductase **Glycerol dehydrogenase **Propanediol-phosphate dehydrogenase ** glycerol-3-phosphate dehydrogenase (NAD+) ** D-xylulose reductase **L-xylulose reductase **Lactate dehydrogenase **Malate dehydrogenase **Isocitrate dehydrogenase ** HMG-CoA reductase * :EC 1.1.2 (with a cytochrome as acceptor) * :EC 1.1.3 (with oxygen as acceptor) **Glucose oxidase **L-gulonolactone oxidase **Thiamine oxidase **Xanthine oxidase * :EC 1.1.4 (with a disul ...
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Catalysis
Catalysis () is the process of increasing the rate of a chemical reaction by adding a substance known as a catalyst (). Catalysts are not consumed in the reaction and remain unchanged after it. If the reaction is rapid and the catalyst recycles quickly, very small amounts of catalyst often suffice; mixing, surface area, and temperature are important factors in reaction rate. Catalysts generally react with one or more reactants to form intermediates that subsequently give the final reaction product, in the process of regenerating the catalyst. Catalysis may be classified as either homogeneous, whose components are dispersed in the same phase (usually gaseous or liquid) as the reactant, or heterogeneous, whose components are not in the same phase. Enzymes and other biocatalysts are often considered as a third category. Catalysis is ubiquitous in chemical industry of all kinds. Estimates are that 90% of all commercially produced chemical products involve catalysts at some s ...
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Bacillus Subtilis
''Bacillus subtilis'', known also as the hay bacillus or grass bacillus, is a Gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges. As a member of the genus ''Bacillus'', ''B. subtilis'' is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. ''B. subtilis'' has historically been classified as an obligate aerobe, though evidence exists that it is a facultative anaerobe. ''B. subtilis'' is considered the best studied Gram-positive bacterium and a model organism to study bacterial chromosome replication and cell differentiation. It is one of the bacterial champions in secreted enzyme production and used on an industrial scale by biotechnology companies. Description ''Bacillus subtilis'' is a Gram-positive bacterium, rod-shaped and catalase-positive. It was originally named ''Vibrio subtilis'' by Christian Gottfried Ehrenberg, and renamed ''B ...
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Aspergillus Aculeatus
''Aspergillus aculeatus'' is a fungus species in the genus ''Aspergillus''. It has been implicated as the causative agent in plant disease. ''A. aculeatus'' belongs to the group of black ''Aspergilli'' which are important industrial workhorses.Pariza, M. W.; Foster, E. M. Determining the Safety of Enzymes Used in Food Processing. ''A. aculeatus'' belongs to the ''Nigri'' section. ''Aspergillus aculeatus'' is considered to be a ubiquitous species that could be usually isolated from rotting fruits and soil. Morphological characteristics such a color, size, shape and ornamentation of conidia are crucial for the classification of strains of black-spored ''Aspergillu''s species. Modern biochemical and molecular identification techniques are helpful in the identification of ''Aspergillus'' isolates, as black-spored ''Aspergillus'' species may have significant variations in their morphological and physiological characteristics. ''Aspergillus'' can rapidly degrade cell walls of plants t ...
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