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Resolution (chromatography)
In chromatography, resolution is a measure of the separation of two Summit (topography), peaks of different retention time ''t'' in a chromatogram. Expression Chromatographic peak resolution is given by :R_s = 2\cfrac where tR is the retention time and wb is the peak width at baseline. Here compound 1 elutes before compound 2. If the peaks have the same width :R_s = \cfrac . Plate number The theoretical plate height is given by :H = \frac where L is the column length and N the number of theoretical plates. The relation between plate number and peak width at the base is given by :N = 16 \cdot \left(\frac\right)^2 \,. See also *Van Deemter equation *Resolution (mass spectrometry) *Image resolution References External linksIUPAC Nomenclature for Chromatography {{chromatography, Chromatography, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatography
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The purpose of preparativ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatogram In English
In chemical analysis, chromatography is a laboratory technique for the Separation process, separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The pu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Summit (topography)
A summit is a point on a surface that is higher in elevation than all points immediately adjacent to it. The topographic terms acme, apex, peak (mountain peak), and zenith are synonymous. The term (mountain top) is generally used only for a mountain peak that is located at some distance from the nearest point of higher elevation. For example, a big, massive rock next to the main summit of a mountain is not considered a summit. Summits near a higher peak, with some prominence or isolation, but not reaching a certain cutoff value for the quantities, are often considered ''subsummits'' (or ''subpeaks'') of the higher peak, and are considered part of the same mountain. A pyramidal peak is an exaggerated form produced by ice erosion of a mountain top. Summit may also refer to the highest point along a line, trail, or route. The highest summit in the world is Mount Everest with a height of above sea level. The first official ascent was made by Tenzing Norgay and Sir Edmund Hillary ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Retention Time
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The purpose of preparativ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatogram
In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the ''stationary phase'' is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and the stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation. Chromatography may be preparative or analytical. The purpose of preparativ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Theoretical Plate
A theoretical plate in many separation processes is a hypothetical zone or stage in which two phases, such as the liquid and vapor phases of a substance, establish an equilibrium with each other. Such equilibrium stages may also be referred to as an equilibrium stage, ideal stage, or a theoretical tray. The performance of many separation processes depends on having series of equilibrium stages and is enhanced by providing more such stages. In other words, having more theoretical plates increases the efficiency of the separation process be it either a distillation, absorption, chromatographic, adsorption or similar process. Applications The concept of theoretical plates and trays or equilibrium stages is used in the design of many different types of separation. Distillation columns The concept of theoretical plates in designing distillation processes has been discussed in many reference texts. Any physical device that provides good contact between the vapor and liquid phases prese ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Van Deemter Equation
The van Deemter equation in chromatography, named for Jan van Deemter, relates the variance per unit length of a separation column to the linear mobile phase velocity by considering physical, kinetic, and thermodynamic properties of a separation. These properties include pathways within the column, diffusion ( axial and longitudinal), and mass transfer kinetics between stationary and mobile phases. In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column. Alternatively, the linear velocity can be taken as the ratio of the column length to the dead time. If the mobile phase is a gas, then the pressure correction must be applied. The variance per unit length of the column is taken as the ratio of the co ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Resolution (mass Spectrometry)
In mass spectrometry, resolution is a measure of the ability to distinguish two peaks of slightly different mass-to-charge ratios ''ΔM'', in a mass spectrum. Resolution and resolving power There are two different definitions of resolution and resolving power in mass spectrometry. IUPAC definition The IUPAC definition for resolution in mass spectrometry is :R = \cfrac = \mathrm :\Delta M = \mathrm :M = \mathrm Where a larger resolution indicates a better separation of peaks. This definition is used in a number of mass spectrometry texts. This use is also implied by the term "high-resolution mass spectrometry." A high value for resolution corresponding to good separation of peaks is similar to the convention used with chromatography separations, although it is important to note that the definitions are not the same. High resolution indicating better peak separation is also used in ion mobility spectrometry. Resolving power definition Some mass spectrometrists use the definit ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Image Resolution
Image resolution is the detail an image holds. The term applies to digital images, film images, and other types of images. "Higher resolution" means more image detail. Image resolution can be measured in various ways. Resolution quantifies how close lines can be to each other and still be visibly ''resolved''. Resolution units can be tied to physical sizes (e.g. lines per mm, lines per inch), to the overall size of a picture (lines per picture height, also known simply as lines, TV lines, or TVL), or to angular subtense. Instead of single lines, line pairs are often used, composed of a dark line and an adjacent light line; for example, a resolution of 10 lines per millimeter means 5 dark lines alternating with 5 light lines, or 5 line pairs per millimeter (5 LP/mm). Photographic lens and film resolution are most often quoted in line pairs per millimeter. Types The resolution of digital cameras can be described in many different ways. Pixel count The term ''resolution'' is o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
