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Photoactivatable Fluorescent Protein
Photoactivatable fluorescent proteins (PAFPs) is a type of fluorescent protein that exhibit fluorescence that can be modified by a light-induced chemical reaction. History The first PAFP, Kaede (protein), was isolated from '' Trachyphyllia geoffroyi'' in a cDNA library screen designed to identify new fluorescent proteins. A fluorescent green protein derived from this screen was serendipitously discovered to have sensitivity to ultraviolet light-- We happened to leave one of the protein aliquots on the laboratory bench overnight. The next day, we found that the protein sample on the bench had turned red, whereas the others that were kept in a paper box remained green. Although the sky had been partly cloudy, the red sample had been exposed to sunlight through the south-facing windows. Properties Many PAFPs have been engineered from existing fluorescent proteins or identified from large-scale screens in the wake of Kaede's discovery. Many of these undergo green-to-red ph ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescent Protein
Fluorescent proteins include: * Green fluorescent protein (GFP) * Yellow fluorescent protein Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish '' Aequorea victoria''. Its excitation peak is 513 nm and its emission peak is 527 nm. Like the parent GFP, YFP ... (YFP) * Red fluorescent protein (RFP) {{Short pages monitor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photochemistry
Photochemistry is the branch of chemistry concerned with the chemical effects of light. Generally, this term is used to describe a chemical reaction caused by absorption of ultraviolet (wavelength from 100 to 400 nm), visible light (400–750 nm) or infrared radiation (750–2500 nm). In nature, photochemistry is of immense importance as it is the basis of photosynthesis, vision, and the formation of vitamin D with sunlight. Photochemical reactions proceed differently than temperature-driven reactions. Photochemical paths access high energy intermediates that cannot be generated thermally, thereby overcoming large activation barriers in a short period of time, and allowing reactions otherwise inaccessible by thermal processes. Photochemistry can also be destructive, as illustrated by the photodegradation of plastics. Concept Grotthuss–Draper law and Stark-Einstein law Photoexcitation is the first step in a photochemical process where the reactant is elevated ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kaede (protein)
Kaede is a photoactivatable fluorescent protein naturally originated from a stony coral, '' Trachyphyllia geoffroyi''. Its name means "maple" in Japanese. With the irradiation of ultraviolet light (350–400 nm), Kaede undergoes irreversible photoconversion from green fluorescence to red fluorescence. Kaede is a homotetrameric protein with the size of 116 kDa. The tetrameric structure was deduced as its primary structure is only 28 kDa. This tetramerization possibly makes Kaede have a low tendency to form aggregates when fused to other proteins. Discovery The property of photoconverted fluorescence Kaede protein was serendipitously discovered and first reported by Ando et al. in Proceedings of the United States National Academy of Sciences. An aliquot of Kaede protein was discovered to emit red fluorescence after being left on the bench and exposed to sunlight. Subsequent verification revealed that Kaede, which is originally green fluorescent, after exposure to UV light i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Trachyphyllia Geoffroyi
The open brain coral (''Trachyphyllia geoffroyi'') is a brightly colored free-living coral species in the family Merulinidae. It is the only species in the monotypic genus ''Trachyphyllia'' and can be found throughout the Indo-Pacific. Description Open brain corals can be solitary or colonial. They are small corals, rarely reaching over 20 cm in diameter. They are free-living and exhibit a flabello-meandroid growth form, meaning they have distinct valley regions separated by walls. In colonial forms, the valley regions can contain multiple individual polyps. Complexity of valley regions can range; some are hourglass shaped while other cans be highly lobed. They typically have bilateral symmetry. During the day when the polyp is closed, the coral is covered by a mantle that extends beyond the skeleton, but can retract when disturbed. Polyps and mantle are very fleshy. Colonies can be blue, green, yellow, brown, and are often vibrantly colored. The open brain coral is know ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
