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Psychrophiles
Psychrophiles or cryophiles (adj. ''psychrophilic'' or ''cryophilic'') are extremophilic organisms that are capable of growth and reproduction in low temperatures, ranging from to . They have an optimal growth temperature at . They are found in places that are permanently cold, such as the polar regions and the deep sea. They can be contrasted with thermophiles, which are organisms that thrive at unusually high temperatures, and mesophiles at intermediate temperatures. Psychrophile is Greek for 'cold-loving', . Many such organisms are bacteria or archaea, but some eukaryotes such as lichens, snow algae, phytoplankton, fungi, and wingless midges, are also classified as psychrophiles. Biology Habitat The cold environments that psychrophiles inhabit are ubiquitous on Earth, as a large fraction of the planetary surface experiences temperatures lower than 10 °C. They are present in permafrost, polar ice, glaciers, snowfields and deep ocean waters. These organisms can also be ...
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Pseudomonas
''Pseudomonas'' is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing 191 described species. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Their ease of culture ''in vitro'' and availability of an increasing number of ''Pseudomonas'' strain genome sequences has made the genus an excellent focus for scientific research; the best studied species include ''P. aeruginosa'' in its role as an opportunistic human pathogen, the plant pathogen '' P. syringae'', the soil bacterium '' P. putida'', and the plant growth-promoting ''P. fluorescens, P. lini, P. migulae'', and ''P. graminis''. Because of their widespread occurrence in water and plant seeds such as dicots, the pseudomonads were observed early in the history of microbiology. The generic name ''Pseudomonas'' created for these organisms was defined in rather vague terms by Walter Migula i ...
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Halomonas
''Halomonas'' is a genus of halophilic (salt-tolerating) bacteria. It grows over the range of 5 to 25% NaCl. The type species of this genus is '' Halomonas elongata''. Description Members of ''Halomonas'' are Gram-negative, rod-shaped bacteria, generally 0.6-0.8 μm by 1.6-1.9 μm. They move by using flagella. They grow in the presence of oxygen, although some have been reported to be able to grow without oxygen. When grown on an agar plate, they form white/yellow colonies that turn light brown over time. Ecology ''Halomonas'' species have been found in a broad variety of saline environments, including estuaries, the ocean, and saline lakes. Species Many species of ''Halomonas'' have been described: '' H. alimentaria'' '' H. alkaliantarctica'' '' H. alkaliphila'' '' H. almeriensis'' '' H. andesensis'' '' H. anticariensis'' '' H. aquamarina'' '' H. arcis'' '' H. axialensis'' '' H. beimenensis'' '' H. boliviensis'' '' H. campaniensis'' '' H. campisalis'' '' H. caseinilytica'' ' ...
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Psychrobacter
''Psychrobacter'' is a genus of Gram-negative, osmotolerant, oxidase-positive, psychrophilic or psychrotolerant, aerobic bacteria which belong to the family Moraxellaceae and the class Gammaproteobacteria. The shape is typically cocci or coccobacilli. Some of those bacteria were isolated from humans and can cause humans infections such as endocarditis and peritonitis. This genus of bacteria is able to grow at temperatures between −10 and 42 °C. Rudi Rossau found through DNA-rRNA hybridization analysis that ''Psychrobacter'' belongs to the Moraxellaceae. The first species (''Psychrobacter immobilis'') was described by Juni and Heym. ''Psychrobacter'' occur in wide range of moist, cold saline habitats, but they also occur in warm and slightly saline habitats. Hypoacylated lipopolysaccharide (LPS) isolated from '' Psychrobacter cryohalolentis'' and '' Psychrobacter arcticus'' induces moderate TLR4-mediated inflammatory response in macrophages Macrophages (abbreviated ...
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Arthrobacter
''Arthrobacter'' (from the Greek, "jointed small stick”) is a genus of bacteria that is commonly found in soil. All species in this genus are Gram-positive obligate aerobes that are rods during exponential growth and cocci in their stationary phase. ''Arthrobacter'' have a distinctive method of cell division called "snapping division" or reversion in which the outer bacterial cell wall ruptures at a joint. Description ''Arthrobacter'' can be grown on mineral salts pyridone broth, where colonies have a greenish metallic center on incubated at . Under the microscope, ''Arthrobacter'' appear as rods when rapidly dividing, and cocci when in stationary phase. Dividing cells may also appear as chevrons ("V" shapes). Other notable characteristics are that it can use pyridone as its sole carbon source, and that its cocci are resistant to desiccation and starvation. Use in industry ''Arthrobacter'', like other bacterial genera including ''Brevibacterium'', ''Microbacterium'', and '' ...
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Mesophile
A mesophile is an organism that grows best in moderate temperature, neither too hot nor too cold, with an optimum growth range from . The optimum growth temperature for these organisms is 37°C. The term is mainly applied to microorganisms. Organisms that prefer extreme environments are known as extremophiles. Mesophiles have diverse classifications, belonging to two domains: Bacteria, Archaea, and to kingdom Fungi of domain Eucarya. Mesophiles belonging to the domain Bacteria can either be gram-positive or gram-negative. Oxygen requirements for mesophiles can be aerobic or anaerobic. There are three basic shapes of mesophiles: coccus, bacillus, and spiral. Habitat The habitats of mesophiles can include cheese and yogurt. They are often included during fermentation of beer and wine making. Since normal human body temperature is 37 °C, the majority of human pathogens are mesophiles, as are most of the organisms comprising the human microbiome. Mesophiles vs. extremophiles Meso ...
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Permafrost
Permafrost is ground that continuously remains below 0 °C (32 °F) for two or more years, located on land or under the ocean. Most common in the Northern Hemisphere, around 15% of the Northern Hemisphere or 11% of the global surface is underlain by permafrost, with the total area of around 18 million km2. This includes substantial areas of Alaska, Greenland, Canada and Siberia. It can also be located on mountaintops in the Southern Hemisphere and beneath ice-free areas in the Antarctic. Permafrost does not have to be the first layer that is on the ground. It can be from an inch to several miles deep under the Earth's surface. It frequently occurs in ground ice, but it can also be present in non-porous bedrock. Permafrost is formed from ice holding various types of soil, sand, and rock in combination. Permafrost contains large amounts of biomass and decomposed biomass that has been stored as methane and carbon dioxide, making tundra soil a carbon sink. As global war ...
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Viable But Nonculturable
Viable but nonculturable (VBNC) bacteria refers as to bacteria that are in a state of very low metabolic activity and do not divide, but are alive and have the ability to become culturable once resuscitated. Bacteria in a VBNC state cannot grow on standard growth media, though flow cytometry can measure the viability of the bacteria. Bacteria can enter the VBNC state as a response to stress, due to adverse nutrient, temperature, osmotic, oxygen, and light conditions. The cells that are in the VBNC state are morphologically smaller, and demonstrate reduced nutrient transport, rate of respiration, and synthesis of macromolecules. Sometimes, VBNC bacteria can remain in that state for over a year. It has been shown that numerous pathogens and non-pathogens can enter the VBNC state, and therefore it has significant implications in pathogenesis, bioremediation, and other branches of microbiology. The existence of the VBNC state is controversial. The validity and interpretation of the as ...
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Antifreeze Proteins
Antifreeze proteins (AFPs) or ice structuring proteins refer to a class of polypeptides produced by certain animals, plants, fungi and bacteria that permit their survival in temperatures below the freezing point of water. AFPs bind to small ice crystals to inhibit the growth and recrystallization of ice that would otherwise be fatal. There is also increasing evidence that AFPs interact with mammalian cell membranes to protect them from cold damage. This work suggests the involvement of AFPs in cold acclimatization. Non-colligative properties Unlike the widely used automotive antifreeze, ethylene glycol, AFPs do not lower freezing point in proportion to concentration. Rather, they work in a noncolligative manner. This phenomenon allows them to act as an antifreeze at concentrations 1/300th to 1/500th of those of other dissolved solutes. Their low concentration minimizes their effect on osmotic pressure. The unusual properties of AFPs are attributed to their selective af ...
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Carotenoid
Carotenoids (), also called tetraterpenoids, are yellow, orange, and red organic compound, organic pigments that are produced by plants and algae, as well as several bacteria, and Fungus, fungi. Carotenoids give the characteristic color to pumpkins, carrots, parsnips, maize, corn, tomatoes, Domestic Canary, canaries, flamingos, salmon, lobster, shrimp, and daffodils. Carotenoids can be produced from Lipid, fats and other basic organic metabolic building blocks by all these organisms. It is also produced by Endosymbiont, endosymbiotic bacteria in Whitefly, whiteflies. Carotenoids from the diet are stored in the fatty tissues of animals, and exclusively Carnivore, carnivorous animals obtain the compounds from animal fat. In the human diet, Small intestine#Absorption, absorption of carotenoids is improved when consumed with fat in a meal. Cooking carotenoid-containing vegetables in oil and shredding the vegetable both increase carotenoid bioavailability. There are over 1,100 known c ...
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Unsaturated Fatty Acids
In nutrition science, nutrition, biology, and chemistry, fat usually means any ester of fatty acids, or a mixture of such chemical compound, compounds, most commonly those that occur in living beings or in food. The term often refers specifically to triglycerides (triple esters of glycerol), that are the main components of vegetable oils and of adipose tissue, fatty tissue in animals; or, even more narrowly, to triglycerides that are solid or semisolid at room temperature, thus excluding oils. The term may also be used more broadly as a synonym of lipid—any substance of biological relevance, composed of carbon, hydrogen, or oxygen, that is insoluble in water but soluble in non-polar solvents. In this sense, besides the triglycerides, the term would include several other types of compounds like monoglyceride, mono- and diglycerides, phospholipids (such as lecithin), sterols (such as cholesterol), waxes (such as beeswax), and free fatty acids, which are usually present in ...
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Vitrification
Vitrification (from Latin ''vitreum'', "glass" via French ''vitrifier'') is the full or partial transformation of a substance into a glass, that is to say, a non-crystalline amorphous solid. Glasses differ from liquids structurally and glasses possess a higher degree of connectivity with the same Hausdorff dimensionality of bonds as crystals: dimH = 3. In the production of ceramics, vitrification is responsible for its impermeability to water. Vitrification is usually achieved by heating materials until they liquidize, then cooling the liquid, often rapidly, so that it passes through the glass transition to form a glassy solid. Certain chemical reactions also result in glasses. In terms of chemistry, vitrification is characteristic for amorphous materials or disordered systems and occurs when bonding between elementary particles (atoms, molecules, forming blocks) becomes higher than a certain threshold value. Thermal fluctuations break the bonds; therefore, the low ...
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Desiccation
Desiccation () is the state of extreme dryness, or the process of extreme drying. A desiccant is a hygroscopic (attracts and holds water) substance that induces or sustains such a state in its local vicinity in a moderately sealed container. Industry Desiccation is widely employed in the oil and gas industry. These materials are obtained in a hydrated state, but the water content leads to corrosion or is incompatible with downstream processing. Removal of water is achieved by cryogenic condensation, absorption into glycols, and absorption onto desiccants such as silica gel. Laboratory A desiccator is a heavy glass or plastic container, now somewhat antiquated, used in practical chemistry for drying or keeping small amounts of materials very dry. The material is placed on a shelf, and a drying agent or ''desiccant'', such as dry silica gel or anhydrous sodium hydroxide, is placed below the shelf. Often some sort of humidity indicator is included in the desiccator to show, ...
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